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Src suppressed C kinase substrate regulates the lipopolysaccharide-induced TNF-α biosynthesis in rat astrocytes

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Abstract

The protein kinase C (PKC) is known to be a critical component in the signaling cascades that lead to astrocyte-activation. To further understand the mechanism of PKC signaling in astrocyte-activation, we investigated the effect of SSeCKS, a PKC substrate, on LPS-induced cytokine expression in astrocytes by RT-PCR and enzymelinked immunosorbent assay. Exposure of the cells to LPS induced rapid translocation of SSeCKS to the perinuclear sides, ERK activation and pronounced TNF-α production, which can be inhibited by the PKC inhibitor Gö6983. By using siRNA knockdown of SSeCKS expression, LPS-induced signaling events were partly inhibited, including ERK activation, inducible TNF-α biosynthesis and secretion. These results suggest that SSeCKS is involved in the LPS-induced TNF-α expression in astrocytes mediated by PKC.

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Authors and Affiliations

  1. Department of Immunology and Microbiology, Medical College of Nantong University, 226001, Nantong, People’s Republic of China

    L. -l. Sun, C. Cheng, H. -o. Liu, C. -c. Shen, F. **ao, J. Qin & J. -l. Yang

  2. The Jiangsu Province Key Lab of Neuroregeneration, Nantong University, 226001, Nantong, People’s Republic of China

    A. -g. Shen

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  1. L. -l. Sun
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  2. C. Cheng
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  3. H. -o. Liu
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  4. C. -c. Shen
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Correspondence to A. -g. Shen.

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Lin-lin Sun and Chun Cheng contributed equally to this work.

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Sun, L.l., Cheng, C., Liu, H.o. et al. Src suppressed C kinase substrate regulates the lipopolysaccharide-induced TNF-α biosynthesis in rat astrocytes. J Mol Neurosci 32, 16–24 (2007). https://doi.org/10.1007/s12031-007-0003-x

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  • DOI: https://doi.org/10.1007/s12031-007-0003-x

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