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Protective Effects of Selenium on Cadmium-Induced Brain Damage in Chickens

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Abstract

Selenium (Se) is an important dietary micronutrient with antioxidative roles. Cadmium (Cd), a ubiquitous environmental pollutant, is known to cause brain lesion in rats and humans. However, little is reported about the deleterious effects of subchronic Cd exposure on the brain of poultry and the protective roles on the brain by Se against Cd. The aim of this study was to investigate the protective effects of Se on Cd-induced brain damage in chickens. One hundred twenty 100-day-old chickens were randomly assigned to four groups and were fed a basal diet, or Se (as 10 mg Na2SeO3/kg dry weight of feed), Cd (as 150 mg CdCl2/kg dry weight of feed), or Cd + Se in their basic diets for 60 days. Then, concentrations of Cd and Se, production of nitric oxide (NO), messenger RNA (mRNA) level and activity of inducible NO synthase (iNOS), level of oxidative stress, and histological and ultrastructural changes of the cerebrum and cerebellum were examined. The results showed that Cd exposure significantly increased Cd accumulation, NO production, iNOS activities, iNOS mRNA level, and MDA content in the cerebrum and cerebellum. Cd treatment obviously decreased Se content and antioxidase activities and caused histopathological changes in the cerebrum and cerebellum. Se supplementation during dietary Cd obviously reduced Cd accumulation, NO production, mRNA level and activity of iNOS, oxidative stress, and histopathological damage in the cerebrum and cerebellum of chickens. It indicated that Se ameliorates Cd-induced brain damage in chickens by regulating iNOS-NO system changes, and oxidative stress induced by Cd and Se can serve as a potential therapeutic for Cd-induced brain lesion of chickens.

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Acknowledgments

The authors thank the members of the Veterinary Internal Medicine Laboratory in the College of Veterinary Medicine, Northeast Agricultural University, for the help they supplied in the research.

Conflict of Interest

All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Pharmacy, Heilongjiang University of Chinese Medicine, Number 24, He** Road, **angfang District, Harbin, 150040, People’s Republic of China

    Li-li Liu

  2. College of Veterinary Medicine, Northeast Agricultural University, Number 59, Mucai Street, **angfang District, Harbin, 150030, People’s Republic of China

    Li-li Liu, Cheng-ming Li, Zi-wei Zhang, Jiu-li Zhang, Hai-dong Yao & Shi-wen Xu

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Correspondence to Shi-wen Xu.

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Li-li Liu and Cheng-ming Li contributed equally to this work.

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Liu, Ll., Li, Cm., Zhang, Zw. et al. Protective Effects of Selenium on Cadmium-Induced Brain Damage in Chickens. Biol Trace Elem Res 158, 176–185 (2014). https://doi.org/10.1007/s12011-014-9919-5

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