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Kinetics and Thermodynamics of Ethanol Production by Saccharomyces cerevisiae MLD10 Using Molasses

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Abstract

In this study, we have used ultraviolet (UV) and γ-ray induction to get a catabolite repression resistant and thermotolerant mutant with enhanced ethanol production along with optimization of sugar concentration and temperature of fermentation. Classical mutagenesis in two consecutive cycles of UV- and γ-ray-induced mutations evolved one best catabolite-resistant and thermotolerant mutant Saccharomyces cerevisiae MLD10 which showed improved ethanol yield (0.48 ± 0.02 g g−1), theoretical yield (93 ± 3 %), and extracellular invertase productivity (1,430 ± 50 IU l−1 h−1), respectively, when fermenting 180 g sugars l−1 in molasses medium at 43 °C in 300 m3 working volume fermenter. Ethanol production was highly dependent on invertase production. Enthalpy (ΔH*) (32.27 kJ M−1) and entropy (ΔS*) (−202.88 J M−1 K−1) values at 43 °C by the mutant MLD10 were significantly lower than those of β-glucosidase production by a thermophilic mutant derivative of Thermomyces lanuginosus. These results confirmed the enhanced production of ethanol and invertase by this mutant derivative. These studies proved that mutant was significantly improved for ethanol production and was thermostable in nature. Lower fermentation time for ethanol production and maintenance of ethanol production rates (3.1 g l−1 h−1) at higher temperature (43 °C) by this mutant could decrease the overall cost of fermentation process and increase the quality of ethanol production.

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Acknowledgments

The authors would like to thank the University of Agriculture, Faisalabad, 38040, Pakistan, and Shakarganj Mills Ltd., Jhang, Pakistan for providing financial support to carry out this project.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, 38040, Pakistan

    Muhammad Arshad, Sibtain Ahmed & Muhammad Anjum Zia

  2. University of Veterinary and Animal Sciences Lahore (Sub-campus Jhang), Lahore, Pakistan

    Muhammad Arshad

  3. University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA

    Sibtain Ahmed

  4. Government College (GC) University, Allama Iqbal Road, Faisalabad, 38000, Pakistan

    Muhammad Ibrahim Rajoka

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  1. Muhammad Arshad
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  2. Sibtain Ahmed
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  3. Muhammad Anjum Zia
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  4. Muhammad Ibrahim Rajoka
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Correspondence to Sibtain Ahmed or Muhammad Ibrahim Rajoka.

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Arshad, M., Ahmed, S., Zia, M.A. et al. Kinetics and Thermodynamics of Ethanol Production by Saccharomyces cerevisiae MLD10 Using Molasses. Appl Biochem Biotechnol 172, 2455–2464 (2014). https://doi.org/10.1007/s12010-013-0689-x

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  • DOI: https://doi.org/10.1007/s12010-013-0689-x

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