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Production of Galanthamine by Leucojum aestivum Shoots Grown in Different Bioreactor Systems

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Abstract

The production of galanthamine by shoots of Leucojum aestivum grown in different bioreactor systems (shaking and nonshaking batch culture, temporary immersion system, bubble bioreactor, continuous and discontinuous gassing bioreactor) under different culture conditions was studied. The influence of the nutrient medium, weight of inoculum, and size of bioreactor on both growth and galanthamine production was studied. The maximal yield of galanthamine (19.416 mg) was achieved by cultivating the L. aestivum shoots (10 g of fresh inoculum) in a temporary immersion system in a 1-L bioreactor vessel which was used as an airlift culture vessel, gassing 12 times per day (5 min).

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Abbreviations

AChE:

Acetylcholinesterase

BAP:

Benzylaminopurine

DW:

Dry weight

FW:

Fresh weight

Gal:

Galanthamine

NAA:

Naphtaleneacetic acid

TIS:

Temporary immersion system

References

  1. Bastida, J., Lavilla, R., and Viladomat, F. (2006) In The alkaloids: chemistry and biology (Cordell, G. A., ed.), Elsevier, Amsterdam, pp. 87-179.

  2. Thomsen, T., Zendeh, H., Fisher, J., & Kewitz, H. (1991). Biochemical Pharmacology, 41, 139–141.

    Article  CAS  Google Scholar 

  3. Czollner, L., Frantsits, W., Küenburg, B., Hedenig, V., Fröhlich, J., & Jordis, U. (1998). Tetrahedron Letters, 39, 2087–2088.

    Article  CAS  Google Scholar 

  4. Berkov, S., Georgieva, L., Kondakova, V., Atanassov, A., Viladomat, F., Bastida, J., et al. (2009). Biotechnologica Biotechnic Equations, 23, 1170–1176.

    CAS  Google Scholar 

  5. Diop, M. F., Hehn, A., Ptak, A., Chrétien, F., Doerper, S., Gontier, E., et al. (2007). Phytochemistry Reviews, 6, 137–141.

    Article  CAS  Google Scholar 

  6. Pavlov, A., Berkov, S., Courot, E., Gocheva, T., Tuneva, D., Pandova, B., et al. (2007). Process Biochemistry, 42, 734–739.

    Article  CAS  Google Scholar 

  7. Sellés, M., Viladomat, F., Bastida, J., & Codina, C. (1999). Plant Cell Reports, 18, 646–651.

    Article  Google Scholar 

  8. Bergoñón, S., Codina, C., Bastida, J., Viladomat, F., & Melé, E. (1996). Plant Cell, Tissue and Organ Culture, 45, 191–199.

    Article  Google Scholar 

  9. Colque, R., Viladomat, F., Bastida, J., & Codina, C. (2001). Acta Horticulturae, 560, 267–270.

    CAS  Google Scholar 

  10. Colque, R., Viladomat, F., Bastida, J., & Codina, C. (2004). Planta Medica, 70, 1180–1188.

    Article  CAS  Google Scholar 

  11. Georgieva, L., Berkov, S., Kondakova, V., Bastida, J., Viladomat, F., Atanassov, A., et al. (2007). Zeitschrift für Naturforschung. Section C, 62, 627–635.

    CAS  Google Scholar 

  12. Stanilova, M.I., Molle, E.D., and Yanev, S.G. (2010). In The alkaloids: chemistry and biology (Cordell, G. A. ed), Elsevier, Amsterdam, pp. 167-270.

  13. Berkov, S., Pavlov, A., Georgiev, V., Bastida, J., Burrus, M., Ilieva, M., et al. (2009). Natural Product Communications, 4, 359–364.

    CAS  Google Scholar 

  14. Georgiev, V., Berkov, S., Georgiev, M., Burrus, M., Codina, C., Bastida, J., et al. (2009). Zeitschrift für Naturforschung. Section C, 64, 219–224.

    CAS  Google Scholar 

  15. Ptak, A., El Tahchy, A., Wyzgolik, G., Henry, M., & Laurian-Mattar, D. (2010). Plant Cell, Tissue and Organ Culture, 102, 61–67.

    Article  CAS  Google Scholar 

  16. El Tahchy, A., Bordage, S., Ptak, A., Dupire, F., Barre, E., Guillou, C., et al. (2011). Plant Cell, Tissue and Organ, 106, 381–390.

    Article  Google Scholar 

  17. Pérez-Alonso, N., Wilken, D., Gerth, A., Jähn, A., Nitzsche, H. M., Kerns, G., et al. (2009). Plant Cell, Tissue and Organ, 99, 151–156.

    Article  Google Scholar 

  18. Tapia, A., Cheel, J., Theoduloz, C., Rodríguez, J., Schmeda-Hirschmann, G., Gerth, A., et al. (2007). Zeitschrift für Naturforschung. Section C, 62, 447–457.

    CAS  Google Scholar 

  19. Ivanov, I., Georgiev, V., Georgiev, M., Ilieva, M., & Pavlov, A. (2011). Applied Biochemistry and Biotechnology, 163, 268–277.

    Article  CAS  Google Scholar 

  20. Murashige, T., & Skoog, F. (1962). Physiologia Plantarum, 15, 473–497.

    Article  CAS  Google Scholar 

  21. Sellés, M., Bergoñón, S., Viladomat, F., Bastida, J., & Codina, C. (1997). Plant Cell, Tissue and Organ, 49, 129–136.

    Article  Google Scholar 

  22. Codina, C. (2002). In Medicinal and aromatic plants—industrial Profiles, vol. 21: Narcissus and daffodil. In G. R. Hanks (Ed.), The genus Narcissus (pp. 215–241). London: Taylor & Francis.

    Google Scholar 

Download references

Acknowledgments

The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 219795 and from the Generalitat de Catalunya (ref. 2009 SGR 1060). The authors thank VITROFLORA (Poland) for providing the in vitro plant material.

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Authors and Affiliations

  1. BioPlanta GmbH, Deutscher Platz 5, 04103, Leipzig, Germany

    Anika Schumann, Diana Claus & André Gerth

  2. AgroBioInstitute, 8 Dragan Tzankov Blvd, 1164, Sofia, Bulgaria

    Strahil Berkov

  3. Departament de Productes Naturals, Biologia Vegetal i Edafologia, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028, Barcelona, Catalonia, Spain

    Strahil Berkov, Jaume Bastida & Carles Codina

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  1. Anika Schumann
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  2. Strahil Berkov
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  3. Diana Claus
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  4. André Gerth
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  5. Jaume Bastida
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Correspondence to Anika Schumann.

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Schumann, A., Berkov, S., Claus, D. et al. Production of Galanthamine by Leucojum aestivum Shoots Grown in Different Bioreactor Systems. Appl Biochem Biotechnol 167, 1907–1920 (2012). https://doi.org/10.1007/s12010-012-9743-3

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  • DOI: https://doi.org/10.1007/s12010-012-9743-3

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