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Cloning, Differential Expression, and Association Analysis with Fat Traits of Porcine IDH3γ Gene

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Abstract

Mitochondrial NAD+-dependent isocitrate dehydrogenase (IDH3) catalyzes the allosterically regulated rate-limiting step of the tricarboxylic acid cycle activated. In pigs, very little is known about this gene. Here, we cloned 1,346 bp full-length cDNA and 8,778 bp genomic sequence of porcine γ subunit of IDH3 (IDH3γ). IDH3γ contains 12 exons separated by 11 introns. Real-time PCR revealed that IDH3γ mRNA were upregulated in backfat of Large White compared with Meishan and F1 hybrids, and most abundant in small intestine via tissue distribution profile. A microsatellite (“GT” repeats) in second intron was found. The selected pigs were genotyped at this microsatellite. The IDH3γ genotypes showed a significant effect on backfat thickness at thorax–waist (P < 0.05), backfat thickness at sixth to seventh thorax (P < 0.01), and average backfat thickness (P < 0.05). This site seemed to be significantly dominant in action (P < 0.05 for backfat thickness at sixth to seventh thorax, backfat thickness at thorax–waist, and average backfat thickness), and allele B was associated with increase of thickness values of these traits. This locus is possibly considered as a marker for adipose deposition traits.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (31000996) and International Foundation for Science (B/4534-1), and Specialized Research Fund for the Doctoral Program of Higher Education (200805041012).

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Authors and Affiliations

  1. Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070, China

    Zhuqing Ren, Yuanzhu **ong, Changyan Deng & Siwen Jiang

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  1. Zhuqing Ren
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  2. Yuanzhu **ong
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  3. Changyan Deng
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  4. Siwen Jiang
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Correspondence to Zhuqing Ren.

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Ren, Z., **ong, Y., Deng, C. et al. Cloning, Differential Expression, and Association Analysis with Fat Traits of Porcine IDH3γ Gene. Appl Biochem Biotechnol 166, 1112–1120 (2012). https://doi.org/10.1007/s12010-011-9499-1

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  • DOI: https://doi.org/10.1007/s12010-011-9499-1

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