Abstract
Antioxidative and hepatoprotective activity of a cultured lichen Usnea ghattensis has been studied. The methanolic extract of cultured lichen U. ghattensis showed good antioxidant activity by preventing lipid peroxidation by 67% and 86% in Trolox-equivalent antioxidant capacity at 20 μg/ml. It also showed superoxide, 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, and hydroxyl radical-scavenging activity, 89%, 89.6%, 94.8%, and 89.6%, respectively, and found levels higher then that known for the synthetic antioxidants butylated hydroxytoluene, butylated hydroxyanisol, and quercetin at 20 μg/ml concentration. The cultured lichen extract also showed hepatoprotection against ethanol-induced toxicity in the mice liver slice culture model by a significant decrease in the antioxidant enzymes, glutathione peroxidase, catalase, and superoxide dismutase, along with a decrease in lipid peroxidation and lactate dehydrogenase release.
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Acknowledgments
We are very grateful to the Department of Biotechnology, Government of India, New Delhi, for the financial support (Grant no. BT/PR 3133/BCE/08/237/2002 dated 21.02.2003). We also gratefully acknowledge financial support in the form of a Senior Research Fellowship to Neeraj Verma from Council of Scientific Industrial Research, New Delhi, India.
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Verma, N., Behera, B.C. & Makhija, U. Antioxidant and Hepatoprotective Activity of a Lichen Usnea ghattensis in Vitro. Appl Biochem Biotechnol 151, 167–181 (2008). https://doi.org/10.1007/s12010-008-8164-9
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DOI: https://doi.org/10.1007/s12010-008-8164-9