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Micro- and Nano-CT for the Study of Bone Ultrastructure

  • Imaging (T Lang and F Wehrli, Section Editors)
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Abstract

Micro-computed tomography (micro-CT)—a version of X-ray CT operating at high spatial resolution—has had a considerable success for the investigation of trabecular bone micro-architecture. Currently, there is a lot of interest in exploiting CT techniques at even higher spatial resolutions to assess bone tissue at the cellular scale. After recalling the basic principles of micro-CT, we review the different existing system, based on either standard X-ray tubes or synchrotron sources. Then, we present recent applications of micro- and nano-CT for the analysis of osteocyte lacunae and the lacunar-canalicular network. We also address the question of the quantification of bone ultrastructure to go beyond the sole visualization.

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Acknowledgments

The images of bone samples were acquired at ESRF within the Long Term Project MD431. This work was performed within the framework of the LABEX PRIMES (ANR-11-LABX-0063) of Université de Lyon. The authors also want to thank Felix W. Wehrli for editing the manuscript.

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Conflict of Interest

F. Peyrin, P. Dong, A. Pacureanu, and M. Langer declare that they have no conflicts of interest.

Human and Animal Rights and Informed Consent

All studies by the authors involving animal and/or human subjects were performed after approval by the appropriate institutional review boards. When required, written informed consent was obtained from all participants.

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Authors and Affiliations

  1. INSERM U1044, CREATIS, 69621, Villeurbanne, France

    Françoise Peyrin, Pei Dong & Max Langer

  2. INSA-Lyon, 69621, Villeurbanne, France

    Françoise Peyrin, Pei Dong & Max Langer

  3. CNRS UMR 5220, 69621, Villeurbanne, France

    Françoise Peyrin, Pei Dong & Max Langer

  4. Université de Lyon, 69622, Lyon, France

    Françoise Peyrin, Pei Dong & Max Langer

  5. Université Lyon 1, 69622, Villeurbanne, France

    Françoise Peyrin, Pei Dong & Max Langer

  6. European Synchrotron Radiation Facility, BP 220, 38043, Grenoble, Cedex, France

    Françoise Peyrin, Pei Dong, Alexandra Pacureanu & Max Langer

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  1. Françoise Peyrin
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  2. Pei Dong
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  3. Alexandra Pacureanu
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  4. Max Langer
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Correspondence to Françoise Peyrin.

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Peyrin, F., Dong, P., Pacureanu, A. et al. Micro- and Nano-CT for the Study of Bone Ultrastructure. Curr Osteoporos Rep 12, 465–474 (2014). https://doi.org/10.1007/s11914-014-0233-0

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