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Enhanced l-Dopa production from elicited cell suspension culture of Hybanthus enneaspermus (L.) F. Muell

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Abstract

Hybanthus enneaspermus is an important source for l-Dopa production. This study reports elicited l-Dopa production in cell suspension cultures of Hybanthus enneaspermus. Cell suspension culture was established using green friable calli from leaf explants cultured on MS medium containing 2.0 mg/l 2,4-D. Effects of different elicitors such as SA, YE, MeJA and AgNO3 on biomass accumulation and l-Dopa content were studied. Among the elicitor tested SA treated culture produced highest biomass and l-Dopa according to their exposure time and concentration. Maximum biomass of 15.5 ± 0.16 g FW, 4.05 ± 0.18 g DW and l-Dopa production of 8.88 mg/g DW were observed at 150 μM concentration of SA. This was 9.25-fold higher compared to that of the unelicited control culture. The results obtained in this study clearly show that the elicitation strategy is a promising method for biosynthesis of l-Dopa production by cell suspension cultures of H. enneaspermus.

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Change history

  • 03 October 2019

    In the original publication of the article

Abbreviations

YE:

Yeast extract

SA:

Salicylic acid

MeJA:

Methyl jasmonate

AgNO3 :

Silver nitrate

NAA:

1-Napthalene acetic acid

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

6-Benzylaminopurine

FW:

Fresh weight

DW:

Dry weight

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Acknowledgements

The authors are thankful to University grants commission-Special Assistance Programme (UGC-SAP), New Delhi, Govt. of India for financial assistance Ref: 3-27/2011 (SAP-11) Dt/ March, 2011.

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Authors and Affiliations

  1. Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Selvam Sathish, Venkatachalam Vasudevan, Sivabalan Karthik, Gadamchetty Pavan & Markandan Manickavasagam

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  1. Selvam Sathish
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  2. Venkatachalam Vasudevan
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  3. Sivabalan Karthik
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  4. Gadamchetty Pavan
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Contributions

SS performed experiments, analyzed, compiled data and prepared manuscript. VV helped to carry out HPLC analysis. SK and GP contributed to statistical analysis. MM supervised the findings of this work and critically evaluated the manuscript.

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Correspondence to Markandan Manickavasagam.

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The original version of this article was revised due to the name of the corresponding author was published incorrectly and corrected in this version.

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Sathish, S., Vasudevan, V., Karthik, S. et al. Enhanced l-Dopa production from elicited cell suspension culture of Hybanthus enneaspermus (L.) F. Muell. Plant Biotechnol Rep 13, 613–621 (2019). https://doi.org/10.1007/s11816-019-00555-y

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