Abstract
This communication describes for the first time an efficient and reproducible protocol for large-scale multiplication of Bambusa nutans. Nodal segments collected from field-grown clumps and cultured on Murashige and Skoog (MS) medium supplemented with 4.4 μM benzylaminopurine (BA) and 2.32 μM kinetin (Kin) gelled with 0.2% gelrite yielded 80% aseptic cultures with 100% bud-break. The in vitro-formed shoots obtained after bud-break were successfully multiplied in MS liquid medium supplemented with 13.2 μM BA, 2.32 μM Kin, and 0.98 μM indole-3-butyric acid (IBA). Sub-culturing of shoots every 3 weeks on fresh multiplication medium yielded a consistent proliferation rate of 3.5-fold. Shoot clusters containing three to five shoots were successfully rooted with 100% success on half-strength MS liquid medium supplemented with 9.8 μM IBA, 2.85 μM indole-3-acetic acid (IAA), 2.68 μM naphthaleneacetic acid (NAA), and 3% sucrose. Plantlets grown in vitro were acclimatized and subsequently transferred to the field. Inter-simple sequence repeat analysis has confirmed the genetic uniformity of the tissue-cultured plants up to 27 passages.
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Acknowledgments
Our profound thanks to Dr. S. A. Ansari, Tropical Forest Research Institute (TFRI), Jabalpur, for providing us with research material of B. nutans and to Dr. R. K. Pachauri, Director General, The Energy and Resources Institute (TERI), for infrastructure support. Thanks are due to Dr. Shashi Bhushan Tripathi and Mr. Madan Singh Negi for their help in planning the clonal fidelity experiments. The financial support of Council of Scientific and Industrial Research, Government of India, extended to Ms. Divya Negi is also gratefully acknowledged.
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Negi, D., Saxena, S. In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation. Plant Biotechnol Rep 5, 35–43 (2011). https://doi.org/10.1007/s11816-010-0154-z
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DOI: https://doi.org/10.1007/s11816-010-0154-z