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Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors

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Abstract

Hypericum perforatum L. is a traditional medicinal plant for the treatment of depression and wound healing, and hypericin is one of the main effective active substances. To optimize the culture system for producing hypericin in adventitious root, this study used balloon-type airlift bioreactors to investigate the effect of air volume, inoculation density, indole-3-butyric acid (IBA) concentration and methyl jasmonate (MeJA) concentration on hypericin content and productivity during adventitious root culture. Hypericin content and productivity were improved with increasing air volume, and 0.1 vvm (air volume/culture volume/min) was optimal for hypericin production. Inoculation density also had a great effect on hypericin accumulation. Hypericin content and productivity were favorable in an inoculation density of 5.0 g l−1 and decreased when inoculation densities were lower or higher than 5.0 g l−1. Furthermore, 1.25 mg l−1 IBA enhanced hypericin content and productivity, but too low (≤0.50 mg l−1) or too high (≥1.50 mg l−1) IBA concentrations decreased hypericin accumulation. MeJA concentration significantly affected biomass accumulation and hypericin production. The biomass decreased and hypericin production increased with increasing MeJA concentration. Optimum hypericin content (1.61 mg g−1 DW) and productivity (15.57 mg l−1) were obtained at 350 μM MeJA. The hypericin content in bioreactor-grown adventitious roots was lower than in 3-year field-grown plants, but significantly higher than that in in vitro-grown plantlets and 1-year field-grown plants. Thus, the bioreactor culture of adventitious roots can realize rapid and mass production of hypericin in H. perforatum.

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Abbreviations

vvm:

Air volume/culture volume/min

DW:

Dry weight

FW:

Fresh weight

IBA:

Indole-3-butyric acid

HPLC:

High-performance liquid chromatography

HIV-1:

Human immunodeficiency virus type 1

MeJA:

Methyl jasmonate

MS:

Murashige and Skoog

rpm:

Revolutions per minute

SA:

Salicylic acid

SE:

Standard error

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Acknowledgments

This research was supported by the National Science Foundation of China (81160497 and 31260182). We thank Chungbuk National University for providing experimental materials.

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Authors and Affiliations

  1. Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, Yanji, 133002, Jilin, China

    Song-Quan Wu, **ao-Kun Yu, Mei-Lan Lian & Xuan-Chun Piao

  2. Department of Horticulture Science, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    So-Young Park

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  1. Song-Quan Wu
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  2. **ao-Kun Yu
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  3. Mei-Lan Lian
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  4. So-Young Park
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Correspondence to Mei-Lan Lian or Xuan-Chun Piao.

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Communicated by K.-Y. Paek.

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Wu, SQ., Yu, XK., Lian, ML. et al. Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors. Acta Physiol Plant 36, 975–981 (2014). https://doi.org/10.1007/s11738-013-1476-6

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  • DOI: https://doi.org/10.1007/s11738-013-1476-6

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