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Comparative evaluation of hydro-, chemo- , and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.)

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Abstract

The present study was aimed to evaluate the effect of different seed priming methods to enhance the sodium chloride (NaCl) and polyethylene glycol-8000 (PEG-8000) stress tolerance in Indian mustard (Brassica juncea L.). Seeds subjected to different priming treatments such as water (hydro-priming), calcium chloride (CaCl2) (chemo-priming), and abscisic acid (ABA) (hormonal-priming) showed increased rate of germination as compared to non-primed seeds. The primed and non-primed seeds were grown for 15 days and then the seedlings were independently subjected to iso-osmotic salt (150 mM NaCl) or PEG-8000 (20%) stress. The different biochemical responses were studied 10 days after treatment. Under NaCl and PEG stress, the dry weight and total chlorophyll content were higher in primed sets as compared to non-primed treatment which was also evident by the phenotype of the seedlings. In general, the higher activities of superoxide dismutase and glutathione reductase resulted in lower oxidative damage, in terms of malondialdehyde content, under NaCl and PEG stress in hydro-primed set as compared to non-primed, ABA-, and CaCl2-primed treatments. Besides, the level of total phenolics and accumulation of osmolytes such as free proline, glycine betaine, and total soluble sugars was also lower in hydro-primed set as compared to other primed and non-primed treatments. The study thus suggests the use of hydro-priming as a simple and cost-effective strategy to alleviate the NaCl and PEG induced stress in B. juncea.

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Abbreviations

ABA:

Abscisic acid

ANOVA:

Analysis of variance

CaCl2 :

Calcium chloride

CP:

Seedlings derived from the CaCl2-primed seeds

CRD:

Completely randomized design

DMRT:

Duncan’s multiple range test

EDTA:

Ethylenediaminetetraacetic acid

GR:

Glutathione reductase

HP:

Seedlings derived from the ABA-primed seeds

MDA:

Malondialdehyde

MS:

Murashige and Skoog basal medium

NaCl:

Sodium chloride

NP:

Seedlings derived from the non-primed seeds

PEG:

Polyethylene glycol-8000

SOD:

Superoxide dismutase

TBARS:

Thiobarbituric acid reactive substances

WP:

Seedlings derived from the hydro-primed seeds

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Acknowledgments

The authors would like to thank Dr. S. J. Jambhulkar, NABTD, for providing seed material of TM2. They are also thankful to Dr. Sudhakar Srivastava, NABTD, for giving critical suggestions during manuscript preparation.

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Authors and Affiliations

  1. Functional Plant Biology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center, Trombay, Mumbai, 400085, India

    Ashish Kumar Srivastava, Penna Suprasanna & Stanislaus Francis D’Souza

  2. Defence Institute of Bio-Energy Research (DIBER), Defence Research & Development Organisation (DRDO), Goraparao, PO Arjunpur, Haldwani, Nainital, 263139, Uttarakhand, India

    Vikas Y. Patade

  3. University of Pune, Pune, India

    Vinayak H. Lokhande & Vikas Y. Patade

  4. Department of Agronomy, Faculty of Agriculture, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar, 90245, Indonesia

    Rinaldi Sjahril

Authors
  1. Ashish Kumar Srivastava
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  2. Vinayak H. Lokhande
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  3. Vikas Y. Patade
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  4. Penna Suprasanna
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  6. Stanislaus Francis D’Souza
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Corresponding author

Correspondence to Penna Suprasanna.

Additional information

Communicated by S. Weidner.

A. K. Srivastava, V. H. Lokhande and V. Y. Patade have contributed equally.

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Srivastava, A.K., Lokhande, V.H., Patade, V.Y. et al. Comparative evaluation of hydro-, chemo- , and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.). Acta Physiol Plant 32, 1135–1144 (2010). https://doi.org/10.1007/s11738-010-0505-y

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