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In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

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Abstract

Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram (4-amino-3,5,6-trichloropicolinic acid) at different concentrations of carbohydrate under the same conditions with presence or absence of luminosity. In our experiment, secondary explants were kept in MS medium containing 8.0 mg·L−1 of Picloram for the callus formation. Calluses were transferred in MS medium supplemented with sucrose, fructose and glucose (control, 2%, 4% and 6%). Results show that Picloram induced the callogenesis in leaf sheath. The secondary embryogenesis was formed in yellow-globular callus. The sucrose as carbohydrate source in the absence of light was more efficient to induce rhizogenesis. Glucose was more efficiency in the presence of light. Callogenic induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.

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Authors and Affiliations

  1. Graduate Program in Forest Resources, Department of Forest Sciences, “Luiz de Queiroz” College of Agriculture, University of São Paulo (ESALQ/USP), Avenida Pádua Dias, 11, Piracicaba, São Paulo, Brazil

    Yurika H. Komatsu, Gilvano Ebling Brondani & Antônio Natal Gonçalves

  2. Graduate Program in Biochemistry of Plants, Department of Biological Sciences, “Luiz de Queiroz” College of Agriculture, University of São Paulo (ESALQ/USP), Avenida Pádua Dias, 11, Piracicaba, São Paulo, Brazil

    Katherine Derlene Batagin-Piotto & Marcílio de Almeida

Authors
  1. Yurika H. Komatsu
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  2. Katherine Derlene Batagin-Piotto
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  3. Gilvano Ebling Brondani
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  4. Antônio Natal Gonçalves
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  5. Marcílio de Almeida
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Correspondence to Antônio Natal Gonçalves or Marcílio de Almeida.

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Foundation project: This study is supported by FAPESP (São Paulo Research Foundation, Brazil) and CAPES (Coordination for the Improvement of Higher Level, Brazil).

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Komatsu, Y.H., Batagin-Piotto, K.D., Brondani, G.E. et al. In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides. Journal of Forestry Research 22, 209–215 (2011). https://doi.org/10.1007/s11676-011-0152-1

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