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A rapid protocol for somatic embryogenesis from immature leaflets of groundnut (Arachis hypogaea L.)

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Summary

Plant regeneration via somatic embryogenesis was developed in two groundnut varieties. Somatic embryogenesis was induced from immature leaflets on MS medium with different concentrations of the auxins 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthaleneacetic acid (NAA) in combination with 0.5 mg/l of the cytokinin BA. The highest frequency of somatic embryo formation occurred on MS medium fortified with 20 mg 2,4-D per l. Of the two auxins tested individually 2,4-D was more effective for induction of embryogenesis as well as production of embryos. Embryo development and maturation was achieved on MS medium supplemented with N6-benzyladenine (BA) (0.5–2.0 mg/l) and 2,4-D (0.5 mg/l). Plant conversion frequency from somatic embryos was highest in presence of 2.0 mg BA per l and 0.5 mg NAA per l. The frequency of embryogenesis and plant regeneration was higher in the VRI-2 cultivar than in the other cultivar tested. Regenerated plants were transferred to soil, grown to maturity, and produced viable seeds.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, India

    N. Jayabalan

  2. Biotechnology Division, Rubber Research Institute of India, 686 009, Kottayam, India

    P. Venkatachalam

  3. Plant Physiology Division, Rubber Research Institute of India, 686 009, Kottayam, India

    N. Geetha

  4. Department of Genetics, Osmania University, 500 007, Hyderabad, India

    P. B. Kavi Kishor

  5. Plant Molecular Biology Lab, Wye College, University of London, Wye Keat, TN25 5AH, London, United Kingdom

    M. Thangavelu

Authors
  1. P. Venkatachalam
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  2. P. B. Kavi Kishor
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  3. N. Geetha
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  4. M. Thangavelu
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  5. N. Jayabalan
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Venkatachalam, P., Kavi Kishor, P.B., Geetha, N. et al. A rapid protocol for somatic embryogenesis from immature leaflets of groundnut (Arachis hypogaea L.). In Vitro Cell.Dev.Biol.-Plant 35, 409–412 (1999). https://doi.org/10.1007/s11627-999-0056-3

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  • DOI: https://doi.org/10.1007/s11627-999-0056-3

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