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Detection of Biogenic Silica in Leaf Blade, Leaf Sheath, and Stem of Bermuda Grass (Cynodon dactylon) Using LIBS and Phytolith Analysis

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Abstract

Laser induced breakdown spectroscopy (LIBS) has been used to perform in situ analysis of major and minor elements present in the different parts of the Bermuda grass (Cynodon dactylon). In situ, point detection/analysis of the elements in plants without any sample preparation has been demonstrated. LIBS spectra of the different parts (leaf blade, leaf sheath and stem) of fresh C. dactylon plant have been recorded to study the pattern of silica deposition in its different parts. Atomic lines of Si, Mg, Ca, C, Al, Zn, N, Sr, etc. have been observed in the LIBS spectra of the C. dactylon. A close observation of LIBS spectra of the different parts of the plants shows that silica concentration is greater in leaf blades than leaf sheaths and stems. The results obtained with LIBS analysis are also compared with the number density of phytoliths deposited in different parts of C. dactylon. It is observed that the highest silicified cell frequency is present in leaf blades followed by leaf sheaths and stems which is in close agreement with LIBS analysis.

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Acknowledgements

Financial assistance from the BRNS, BARC, and Mumbai (no. 2009/37/30/BRNS/2063) is gratefully acknowledged. Mr. Durgesh Kumar Tripathi is also grateful to Allahabad University for providing D.Phil. Fellowship under UGC scheme. Dr. Nilesh Kumar Rai is grateful to CSIR for providing RA Fellowship.

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Authors and Affiliations

  1. Department of Botany, University of Allahabad, Allahabad, India

    D. K. Chauhan & D. K. Tripathi

  2. Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad, India

    N. K. Rai & A. K. Rai

Authors
  1. D. K. Chauhan
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  2. D. K. Tripathi
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  3. N. K. Rai
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  4. A. K. Rai
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Correspondence to A. K. Rai.

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Chauhan, D.K., Tripathi, D.K., Rai, N.K. et al. Detection of Biogenic Silica in Leaf Blade, Leaf Sheath, and Stem of Bermuda Grass (Cynodon dactylon) Using LIBS and Phytolith Analysis. Food Biophysics 6, 416–423 (2011). https://doi.org/10.1007/s11483-011-9219-y

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