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Enhanced lateral flow assay with double conjugates for the detection of exosomes

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Abstract

Exosomes are promising biological biomarkers for monitoring a number of diseases, especially cancers. Here, we developed a double gold nanoparticles (GNPs) conjugates based lateral flow assay (D-LFA) for rapidly and sensitively detecting and molecular profiling of exosomes. Based on these two GNPs conjugates, the signal amplification can be achieved without any additional operation. The antibody on the 1st GNPs conjugate could recognize exosomes and form a sandwich format on the test zone. The 2nd GNPs conjugate was designed to bind to the 1st GNPs conjugate to realize signal amplification. This biosensor enabled visual and quantitative detection of exosomes by the accumulation of GNPs on the test zone and showed a low detection limit of 1.3×103 particles/μL, which has been improved 13-fold compared with the normal lateral flow assay. The D-LFA exhibited good sensitivity and reproducibility and has been successfully used for the detection of exosomes in fetal bovine serum, which proved its potential application in practical diagnostics.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21475009, 21475008) and Bei**g Municipal Science & Technology Commission (Z161100000116037).

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Authors and Affiliations

  1. Research Center for Bioengineering and Sensing Technology, Bei**g Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Bei**g, Bei**g, 100083, China

    Tingting Wu, Yuemeng Yang, Yu Cao, Yan Huang, Li-** Xu & Xueji Zhang

  2. CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, CAS Center for Excellence in Nanoscience, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Bei**g, 100190, China

    Shutao Wang

Authors
  1. Tingting Wu
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  2. Yuemeng Yang
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  3. Yu Cao
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  4. Yan Huang
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  5. Li-** Xu
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  6. Xueji Zhang
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  7. Shutao Wang
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Correspondence to Li-** Xu, Xueji Zhang or Shutao Wang.

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Wu, T., Yang, Y., Cao, Y. et al. Enhanced lateral flow assay with double conjugates for the detection of exosomes. Sci. China Chem. 61, 1423–1429 (2018). https://doi.org/10.1007/s11426-018-9305-6

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  • DOI: https://doi.org/10.1007/s11426-018-9305-6

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