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Rhynchosia rufescens AgNPs enhance cytotoxicity by ROS-mediated apoptosis in MCF-7 cell lines

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Abstract

The present study deals with the synthesis of silver nanoparticles (AgNPs) from Rhynchosia rufescens and to evaluate its cytotoxic effect mediated through induced apoptosis. The reduction and cap** of phytoconstituents was confirmed using FTIR demonstrating O–H and C–H stretching at different peaks. The size and the shape of the particle were determined using scanning electron microscopy (SEM) illustrating 1 μm to 100 nm in size and the composition of compounds in the AgNPs were revealed using XRD and EDX. The results of the antioxidant assays revealed that the synthesized AgNPs had significant radical scavenging potential in dose-dependent inhibition with 22–64% for DPPH and 25–41% for ferric reducing antioxidant power assay at the concentrations of 20–100 μg/ml. Further, the synthesized AgNPs demonstrated potent cytotoxic activity against human breast cancer (MCF-7) cell line with an IC50 value of 26 ± 1.0 μg/ml by the MTT assay. Cytotoxicity was confirmed using AO/EtBr and DAPI staining method where nuclear condensation and fragmentation of cancer cells was observed after treatment with nanoparticle. The results were further confirmed by flow cytometry analysis which revealed the occurrence of apoptosis during the S phase in cell cycle exposing the potential of the AgNPs against MCF-7 cancer cell. From the results, we conclude that the synthesized AgNPs from Rhynchosia rufescens exhibited multifunctional properties.

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Acknowledgments

The authors are grateful to the management and Principal of K.S.Rangasamy College of Technology for providing infrastructure to carry out this research work. Authors are thankful to the infrastructure provided by DST-FIST, India, and DBT-STAR Scheme, India.

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Authors and Affiliations

  1. Department of Biotechnology, K.S.Rangasamy College of Technology, K.S.R. Kalvinagar, Tiruchengode, Tamil Nadu, 637 215, India

    Syed Zameer Ahmed Khader, Sidhra Syed Zameer Ahmed, Gayathri Menon Ganesan, Manimaran Vetrivel & Manavalan Sankarappan

  2. Department of Physics, C. Abdul Hakeem College, Melvisharam, Vellore, Tamil Nadu, India

    Mohamed Rafi Mahboob

  3. Department of Zoology, Bharathiar University, Coimbatore, Tamil Nadu, India

    Paulpandi Manickam

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  1. Syed Zameer Ahmed Khader
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Correspondence to Sidhra Syed Zameer Ahmed.

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Khader, S.Z.A., Syed Zameer Ahmed, S., Ganesan, G.M. et al. Rhynchosia rufescens AgNPs enhance cytotoxicity by ROS-mediated apoptosis in MCF-7 cell lines. Environ Sci Pollut Res 27, 2155–2164 (2020). https://doi.org/10.1007/s11356-019-06479-y

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