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Novel Bacillus cereus strain from electrokinetically remediated saline soil towards the remediation of crude oil

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Abstract

A new strain SWH-15 was successfully isolated after initial electrokinetic remediation experiment using the same saline soil sampled from Shengli Oilfield, China. Four methods (morphological and biochemical characteristics, whole-cell fatty acid methyl esters (FAMEs) analysis, 16S rRNA sequence analysis and DNA G + C content and DNA–DNA hybridization analysis) were used to identify the taxonomic status of SWH-15 and confirmed that SWH-15 was a novel species of the Bacillus (B.) cereus group. Then, we assessed the degrading ability of the novel strain SWH-15 to crude oil through a microcosm experiment with four treatments, including control (CK), bioremediation using SWH-15 (Bio), electrokinetic remediation (EK), and combined bioremediation and electrokinetic remediation (Bio + EK). The results showed that the Bio + EK combined remediation treatment was more effective than the CK, Bio, and EK treatments in degrading crude oil contaminants. Bioaugmentation, by addition of the strain SWH-15 had synergistic effect with EK in Bio + EK treatment. Bacterial community analysis showed that electrokinetic remediation alone significantly altered the bacterial community of the saline soil. The addition of the strain SWH-15 alone had a weak effect on the bacterial community. However, the strain SWH-15 boosted the growth of other bacterial species in the metabolic network and weakened the impact of electrical field on the whole bacterial community structure in the Bio + EK treatment.

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Acknowledgments

This work was funded by the Key Research and Development Plan of Shandong Province (No. 2017GSF17118; No. 2017GSF217005), the National Natural Science Foundation of China (No. 41601331), and Taishan Scholars Project (2015).

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Authors and Affiliations

  1. Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Shandong Provincial Key Laboratory of Applied Microbiology, 28789 East **gshi Road, **an, 250103, China

    Yong-chao Gao, Shu-hai Guo, Jia-ning Wang, Wen Zhang & Guan-hong Chen

  2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110164, China

    Yong-chao Gao & Shu-hai Guo

  3. School of Resources and Environment, University of **an, **an, 250022, China

    Hui Wang

  4. Global Centre for Environmental Remediation, University of Newcastle, Callaghan, NSW, 2308, Australia

    Jianhua Du, Yanju Liu & Ravi Naidu

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  1. Yong-chao Gao
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  2. Shu-hai Guo
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Correspondence to Shu-hai Guo.

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Gao, Yc., Guo, Sh., Wang, Jn. et al. Novel Bacillus cereus strain from electrokinetically remediated saline soil towards the remediation of crude oil. Environ Sci Pollut Res 25, 26351–26360 (2018). https://doi.org/10.1007/s11356-018-2495-z

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