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Bacillus safensis with plant-derived smoke stimulates rice growth under saline conditions

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Abstract

Salinity is a worldwide environmental problem of agricultural lands. Smoke and plant growth-promoting bacteria (PGPR) are individually used to improve plant growth, but the combined effects of these have not been studied yet under saline conditions. The combined effect of plant growth-promoting bacteria Bacillus safensis and plant-derived smoke Cymbopogon jwarancusa was studied under different salinity level as 50, 100, and 150 mM on rice (cv. Basmati-385). Smoke dilutions of C. jwarancusa (C-500 and C-1000) and bacterial culture of B. safensis were used to soak seeds for 10 h. It was observed that the salt concentration decreases the germination percentage, vegetative growth, ion contents (K+ and Ca2+), and photosynthetic pigments (Chl “a,” Chl “b,” and carotene) while an increase occurred in Na+, total soluble protein (TSP), proline, total soluble sugar, catalase (CAT), and peroxidase (POD) contents. The combined effect of B. safensis and smoke primed seeds increased the germination percentage, seedling growth, ion contents (K+, Ca2+), and photosynthetic pigments (Chl “a,” Chl “b,” carotene) and reduced the Na+ ion content, total soluble protein, proline content, total soluble sugar, CAT, and POD activity by lowering the drastic effect of salt stress. It was concluded that combined effect of smoke and PGPR is more effective than individual effect.

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Acknowledgements

This study was funded by Higher Education Commission through grant No. 1348.

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Authors and Affiliations

  1. Department of Bioinformatics & Biotechnology, International Islamic University Islamabad, Islamabad, 44000, Pakistan

    Muhammad Hafeez Ullah Khan, Jabar Zaman Khan Khattak & Ismail Din

  2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China

    Muhammad Hafeez Ullah Khan, Shahid Ullah Khan & Shah Fahad

  3. Department of Biotechnology & Genetic engineering, Kohat University of Science & Technology, Kohat, 26000, Pakistan

    Muhammad Jamil & Ijaz Malook

  4. Zhejiang University Hangzhou, Hangzhou, 310058, People’s Republic of China

    Mehmood Jan

  5. College of Horticulture, Northeast Agricultural University Harbin, Heilongjiang, 150030, China

    Shah Saud

  6. College of Agronomy, Northwest Agriculture and Forestry University Yangling, Shaanxi, 712100, China

    Muhammad Kamran

  7. Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

    Hesham Alharby

  8. Department of Agriculture, University of Swabi, Khyber Pakhtunkhwa, Pakistan

    Shah Fahad

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  1. Muhammad Hafeez Ullah Khan
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  2. Jabar Zaman Khan Khattak
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Khan, M.H.U., Khattak, J.Z.K., Jamil, M. et al. Bacillus safensis with plant-derived smoke stimulates rice growth under saline conditions. Environ Sci Pollut Res 24, 23850–23863 (2017). https://doi.org/10.1007/s11356-017-0026-y

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