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EDTA and hydrochloric acid effects on mercury accumulation by Lupinus albus

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Abstract

The efficiency of white lupine (Lupinus albus) to uptake and accumulate mercury from a soil polluted by mining activities was assessed in a pot experiment with chemically assisted phytoextraction. The mobilizing agents tested were ethylenediaminetetracetic acid (EDTA) and hydrochloric acid (HCl). Two doses of each amendment were used (0.5 and 1.0 g of amendment per kg of soil), and unamended pots were used as a control. Addition of HCl to the soil did not negatively affect plant biomass, while the use of EDTA led to a significant decrease in plant growth when compared to that found for non-treated pots, with plants visually showing symptoms of toxicity. The addition of hydrochloric acid increased root, shoot and total plant Hg uptake of white lupine by 3.7 times, 3.1 times and 3.5 times, respectively, in relation to non-amended plants. The greatest efficiency was obtained for the highest HCl dose. EDTA led to higher concentrations of total plant Hg than that found with the control, but, due to the aforementioned decrease in plant biomass, the Hg phytoextraction yield was not significantly increased. These results were attributed to the capability of both amendments to form stable Hg complexes. The concentration of Hg in the water of the soil pores after the phytoextraction experiment was very low for all treatments, showing that risks derived from metal leaching could be partially avoided by using doses and chemicals suitable to the concentration of metal in the soil and plant performance.

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Authors and Affiliations

  1. Department of Chemical Engineering, School of Civil Engineering, University of Castilla-La Mancha, Avenida Camilo José Cela, s/n, 13071, Ciudad Real, Spain

    Luis Rodríguez & Laura Rodríguez-Castellanos

  2. Department of Physical Chemistry, Faculty of Environmental Sciences and Biochemistry, University of Castilla-La Mancha, Avenida Carlos III, s/n, 45071, Toledo, Spain

    Jacinto Alonso-Azcárate

  3. Department of Chemical Engineering, Institute of Chemical and Environmental Technologies (ITQUIMA), University of Castilla-La Mancha, Avenida Camilo José Cela, s/n, 13071, Ciudad Real, Spain

    José Villaseñor

Authors
  1. Luis Rodríguez
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  2. Jacinto Alonso-Azcárate
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  3. José Villaseñor
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  4. Laura Rodríguez-Castellanos
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Correspondence to Luis Rodríguez.

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Responsible editor: Elena Maestri

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Rodríguez, L., Alonso-Azcárate, J., Villaseñor, J. et al. EDTA and hydrochloric acid effects on mercury accumulation by Lupinus albus . Environ Sci Pollut Res 23, 24739–24748 (2016). https://doi.org/10.1007/s11356-016-7680-3

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