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Immobilization of aluminum with mucilage secreted by root cap and root border cells is related to aluminum resistance in Glycine max L

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Abstract

The root cap and root border cells (RBCs) of most plant species produced pectinaceous mucilage, which can bind metal cations. In order to evaluate the potential role of root mucilage on aluminum (Al) resistance, two soybean cultivars differing in Al resistance were aeroponic cultured, the effects of Al on root mucilage secretion, root growth, contents of mucilage-bound Al and root tip Al, and the capability of mucilage to bind Al were investigated. Increasing Al concentration and exposure time significantly enhanced mucilage excretion from both root caps and RBCs, decreased RBCs viability and relative root elongation except roots exposed to 400 μM Al for 48 h in Al-resistant cultivar. Removal of root mucilage from root tips resulted in a more severe inhibition of root elongation. Of the total Al accumulated in root, mucilage accounted 48–72 and 12–27 %, while root tip accounted 22–52 and 73–88 % in Al-resistant and Al-sensitive cultivars, respectively. A 27Al nuclear magnetic resonance spectrum of the Al-adsorbed mucilage showed Al tightly bound to mucilage. Higher capacity to exclude Al in Al-resistant soybean cultivar is related to the immobilization and detoxification of Al by the mucilage secreted from root cap and RBCs.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31101599; 30800705), the Provincial Natural Science Foundation of Zhejiang (Y3110561).

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Authors and Affiliations

  1. College of Geography and Environmental Sciences, Zhejiang Normal University, 688 Yingbin Avenue, **hua, Zhejiang Province, 321004, People’s Republic of China

    Miaozhen Cai

  2. College of Chemistry and Life Sciences, Zhejiang Normal University, **hua, 321004, People’s Republic of China

    Ning Wang, Fangmei Wang, Kun Wu & **ng Du

  3. Bioengineering Institute, **hua College of Vocation and Technology, **hua, 321007, People’s Republic of China

    Chenghua **ng

Authors
  1. Miaozhen Cai
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  2. Ning Wang
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  3. Chenghua **ng
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  4. Fangmei Wang
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  5. Kun Wu
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  6. **ng Du
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Correspondence to Miaozhen Cai.

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Responsible editor: Elena Maestri

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Cai, M., Wang, N., **ng, C. et al. Immobilization of aluminum with mucilage secreted by root cap and root border cells is related to aluminum resistance in Glycine max L. Environ Sci Pollut Res 20, 8924–8933 (2013). https://doi.org/10.1007/s11356-013-1815-6

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