Abstract
Introduction
Acid violet 7 (AV7), mostly used in food, paper, cosmetic, and especially in textile industries, was degraded by Pseudomonas putida mt-2 at concentrations up to 200 mg/l.
Materials and methods
In this study, toxicity of AV7, before and after biodegradation, was evaluated in vivo, in mouse bone marrow, by assessing the percentage of cells bearing different chromosome aberrations, membrane lipid peroxidation, and acetylcholinesterasic activity inhibition. The studies included same conditions for animal treatment, corresponding to increasing doses by intraperitoneal (ip) injection.
Results
Results indicated that AV7 showed a significant ability to induce chromosome aberrations, lipid peroxidation, and acetylcholinesterase inhibitory effect. The toxicity of AV7 increased significantly after static biodegradation with P. putida mt-2 and totally disappeared after shaken incubation. In addition, the toxicity generated by the pure azo dye and the corresponding azoreduction metabolites (4’-aminoacetanilide (4’-AA) and 5-acetamido-2-amino-1-hydroxy-3,6-naphtalene disulfonic acid (5-ANDS)) were compared. 4’-AA and 5-ANDS would be responsible of static biodegradation medium toxicity. The present study demonstrates that P. putida mt-2, incubated under aerobic condition, has a catabolism which enables it to degrade AV7, and especially to completely detoxify the dye mixture.
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References
Ames BN, McCann J, Yamasaki E (1975) Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutat Res 31:347–364
Ben Mansour H, Corroler D, Barillier D, Ghedira K, Chekir L, Mosrati R (2007) Evaluation of genotoxicity and pro-oxidant effect of the azo dyes: acids yellow 17, violet 7 and orange 52, and of their degradation products by Pseudomonas putida mt-2. Food Chem Toxicol 45:1670–1677
Ben Mansour H, Mosrati R, Corroler D, Ghedira K, Bariller D, Chekir L (2009a) Genotoxic and anti-butyrylcholinesterasic activities of acid violet 7 and its biodegradation products. Drug Chem Toxicol 32:230–237
Ben Mansour H, Bariller D, Correler D, Ghedira K, Chekir L, Mosrati R (2009b) In vitro mutagenicity of acid violet 7 and its degradation products by Pseudomonas putida mt-2: correlation with chemical structure. Environ Toxicol Pharmacol 27:231–236
Carita R, Marin-Morales MA (2008) Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes. Chemosphere 72:722–725
Chadwick RW, George SE, Claxton LD (1992) Role of the gastrointestinal mucosa and microflora in the bioactivation of dietary and environmental mutagens or carcinogens. Drug Metabolism Rev 24:425–492
Chang JS, Chou C, Lin Y, Ho J, Hu TL (2001) Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas luteola. Water Res 35:2041–2050
Eldeen IMS, Elgorashi EE, Van Staden J (2005) Antibacterial, anti-inflammatory, anticholinesterase and mutagenic effects of extracts obtained from some trees used in South African traditional medicine. J Ethnopharmacol 102:457–464
Ellman GL, Courtney KD Jr, VA FRM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95
Evans EP, Breckon G, Ford CE (1960) An air drying method for meiotic preparation from mammalian tests. Cytogenetics 3:613–616
Fagali N, Catalá A (2009) Feand Feinitiated peroxidation of sonicated and non-sonicated liposomes made of retinal lipids in different aqueous media. Chem Phys Lipids 159:88–94
Haug W, Schmidt A, Nortemann B, Hempel DC, Stolz A, Knackmuss HJ (1991) Mineralization of the sulfonated azo dye mordant yellow 3 by 6-aminonaphthalene-2sulfonate-degrading bacterial consortium. Appl Environ Microbiol 57:3144–3149
Heiss GS, Gowan B, Dabbs ER (1992) Cloning of DNA from a Rhodococcus strain conferring the ability to decolorize sulfonated azo dyes. FEMS Microbiol Lett 99:221–226
Kurosumi A, Erika K, Nakamura Y (2008) Degradation of reactive dyes by ozonation and oxalic acid-assimilating bacteria isolated from soil. Biodegradation 19:489–494
Küçükkilinç T, Özer I (2007) Multi-site inhibition of human plasma cholinesterase by cationic phenoxazine and phenothiazine dyes. Arch Biochem Biophys 461:294–298
Nakayama T, Kimura T, Kodama Nagata MC (1983) Generation of hydrogen peroxide and superoxide anions from active metabolites of naphthalamines and aminoazo dyes: its possible role in carcinogenesis. Carcinogenesis 4:765–769
Noor A-T, Fatima I, Ahmad I, Malik A, Afza N, Iqbal L, Latif M, Khan SB (2007) Leufolins A and B, potent butyrylcholinesterase-inhibiting flavonoid glucosides from leucas urticifolia. Molecules 12:1447–1454
Ohkowa H, Ohisi N, Yagi K (1979) Assay for lipid peroxides in animals tissue by thiobarbituric acid reaction. Anal Biochem 95:351–358
Ortega MG, Agnese AM, Cabrera JL (2004) Anticholinesterase activity in an alkaloid extract of Huperzia saururus. Phytomedicine 11:539–543
Osman MY, Sharaf AI, Osman MY, El-Khouly AZ, Ahmed IE (2004) Synthetic organic food colouring agents and their degraded products: effects on human and rat cholinesterases. Br J Biomed Sci 61:128–132
Ouanes Z, Ayed-Boussema I, Baati T, Creppy EE, Bacha H (2005) Zearalenone induces chromosome aberrations in mouse bone marrow: preventive effect of 17β-estradiol, progesterone and Vitamin E. Mutat Res 565:139–149
Pandey A, Singh P, Iyengar L (2007) Bacterial decolorization and degradation of azo dyes. Int Biodeterior Biodegrad 59:73–84
Roex EWM, Keijzers R, Van Gestel CAM (2003) Acetylcholinesterase inhibition and increased food consumption rate in the zebrafish, Danio rerio, after chronic exposure to parathion. Aquat Toxicol 64:451–460
Stahlmann R, Wegner M, Riecke K, Kruse M, Platzek T (2006) Sensitising potential of four textile dyes and their metabolites in a modified local lymph node assay. Toxicol 219:113–123
Sweeney EA, Chipman JK, Forsythe SJ (1994) Evidence for direct-acting oxidative genotoxicity by reduction products of azo dyes. Environ Health Perspect 102:119–122
Tahara M, Kubota R, Nakazawa H, Tokunaga H, Nishimura T (2005) Use of cholinesterase activity as an indicator for the effects of combinations of organophosphorus pesticides in water from environmental sources. Water Res 39:5112–5118
Vandevivere PC, Bianchi R, Verstraete W (1998) Treatment and reuse of wastewater from the textile wet-processing industry: review of emerging technologies. J Chem Technol Biotechnol 72:289–302
Wang W (1991) Toxicity assessment of pretreated industrial effluent using higher plant. Res Journal Water Pollution Control Fed 62:853–860
Williams RD, Boros GL, Kolanko CJ, Jackman SM, Eggers TR (2004) Chromosomal aberrations in human lymphocytes exposed to the anticholinesterase pesticide isofenphos with mechanisms of leukemogenesis. Leuk Res 28:947–958
Yosida TH, Amano K (1965) Autosomal polymorphism in laboratory bred and wild Norway rats, Rattus norvegicus. Chromosoma 16:658–667
Zollinger H (1987) Colour chemistry—synthesis, properties and applications of organic dyes and pigments. VCH, New York
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Ben Mansour, H., Ayed-Ajmi, Y., Mosrati, R. et al. Acid violet 7 and its biodegradation products induce chromosome aberrations, lipid peroxidation, and cholinesterase inhibition in mouse bone marrow. Environ Sci Pollut Res 17, 1371–1378 (2010). https://doi.org/10.1007/s11356-010-0323-1
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DOI: https://doi.org/10.1007/s11356-010-0323-1