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Suppression of Fusarium oxysporum and induced resistance of plants involved in the biocontrol of Cucumber Fusarium Wilt by Streptomyces bikiniensis HD-087

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Abstract

Cucumber Fusarium Wilt, caused by Fusarium oxysporum f. sp. cucumerinum, which usually leads to severe economic damage, is a common destructive disease worldwide. To date, no effective method has yet been found to counteract this disease. A fungal isolate, designated HD-087, which was identified as Streptomyces bikiniensis using physiological-biochemical identification and 16S rRNA sequence analysis, is shown to possess distinctive inhibitory activity against F. oxysporum. The fermentation broth of HD-087 leads to certain abnormalities in pathogen hyphae. It peroxidizes cell membrane lipids, which leads to membrane destruction along with cytoplasm leakage. This broth also restrains germination of the conidia. The activities of the enzymes peroxidase, phenylalanine ammonia-lyase, and β-1,3-glucanase in cucumber leaves were dramatically increased after treated with fermentation broth of HD-087. The levels of chlorophyll and soluble sugars were also found to be increased, with the relative conductivity of leaves being reduced. In short, the metabolites of strain HD-087 can effectively suppress F. oxysporum and trigger induced resistance in cucumber.

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Abbreviations

CFW:

Cucumber Fusarium Wilt

POD:

Peroxidase

PAL:

Phenylalanine ammonia-lyase

MDA:

Malondialdehyde

PDA:

Potato dextrose agar

BC:

Broth control

PC:

Pathogen control

UC:

Untreated control

BAP:

Broth root irrigation after pathogen infection

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Acknowledgments

This work was supported by a fellowship from the Key Laboratory of Microbiology, Heilongjiang University, China.

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Authors and Affiliations

  1. State Key Laboratory of Desert and Oasis Ecology, **njiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China

    Shuai Zhao & Chang-Yan Tian

  2. Graduate University of Chinese Academy of Sciences, Bei**g, 100049, China

    Shuai Zhao

  3. Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin, 150000, China

    Chun-Mei Du

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  1. Shuai Zhao
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Correspondence to Chang-Yan Tian.

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Zhao, S., Du, CM. & Tian, CY. Suppression of Fusarium oxysporum and induced resistance of plants involved in the biocontrol of Cucumber Fusarium Wilt by Streptomyces bikiniensis HD-087. World J Microbiol Biotechnol 28, 2919–2927 (2012). https://doi.org/10.1007/s11274-012-1102-6

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  • DOI: https://doi.org/10.1007/s11274-012-1102-6

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