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Polymorphism of fecundity genes (BMP15 and GDF9) and their association with litter size in Bangladeshi prolific Black Bengal goat

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Abstract

Goat farming in Bangladesh is primarily centred on indigenous Black Bengal goat, a highly prolific breed. Searching for genetic markers associated with prolificacy in this breed is vital for the country’s goat breeding industry. However, there are no reports on polymorphisms associated with the fertility of Bangladeshi Black Bengal goats. This study investigated two major fecundity genes-bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) to detect any possible mutations in these two genes associated with litter size in Black Bengal goats. Blood samples were collected from 40 raised goats in Hathazari Government Goat Farm, Bangladesh. Genomic DNA was extracted; PCR amplification was performed; and sequencing of PCR products was performed to detect polymorphism loci in the target genes. Five SNPs viz. C735A, C743A, G754T, C781A and C808G were detected in exon 2 of BMP15 gene. A SNP (T1173A) was detected in GDF9 exon 2. Association results show that SNPs at the 735, 754 and 781 nucleotide positions of BMP15 exon 2 had a significant association with litter size in Black Bengal goat. The effect of parity was also highly significant (P < 0.001) on litter size. For the first time, this study explored SNP loci in fecundity genes in Bangladeshi prolific Black Bengal goats. Further studies with many genetically unrelated animals for assessing the association of these loci and others in the fecundity genes with litter size may be useful.

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Data availability

The data that support the findings of this study are available from the authors upon reasonable request.

References

  • Abdoli, R., Mirhoseini, S.Z., Hossein-Zadeh, N.G. and Zamani, P., 2018. Screening for causative mutations of major prolificacy genes in Iranian fat-tailed sheep. International Journal of Fertility and Sterility, 12, 51-55.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Adzhubei, I., Jordan, D.M. and Sunyaev, S.R., 2013. Predicting functional effect of human missense mutations using PolyPhen-2. Current Protocols in Human Genetics, 07, unit, 7.20.

  • Ahlawat, S., Sharma, R. and Maitra, A., 2013. Screening of indigenous goats for prolificacy associated DNA markers of sheep, Gene, 517, 128-131.

    Article  CAS  Google Scholar 

  • Ahlawat, S., Sharma, R., Roy, M., Tantia, M.S. and Prakash, V., 2015. Association analysis of novel SNPs in BMPR1B, BMP15 and GDF9 genes with reproductive traits in Black Bengal goats. Small Ruminant Research, 132, 92-98.

    Article  Google Scholar 

  • Ahlawat, S., Sharma, R., Roy, M., Mandakmale, S., Prakash, V. and Tantia, M.S., 2016. Genoty** of Novel SNPs in BMPR1B, BMP15, and GDF9 genes for association with prolificacy in seven Indian goat breeds. Animal Biotechnology, 27, 199-207.

    Article  CAS  Google Scholar 

  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J., 1990. Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410.

    Article  CAS  Google Scholar 

  • Amin, M., Husain, S. and Islam, A., 2000. Evaluation of Black Bengal goats and their cross with the Jamunapari breed for carcass characteristics. Small Ruminant Research, 38, 211-215.

    Article  CAS  Google Scholar 

  • Chen, N., Juric, I., Cosgrove, E.J., Bowman, R., Fitzpatrick, J.W., Schoech, S.J., Clark, A.G. and Coop, G., 2019. Allele frequency dynamics in a pedigreed natural population. Proceedings of the National Academy of Sciences of the United States of America, 116, 2158-2164.

    Article  CAS  Google Scholar 

  • Choudhury, M., Sarker, S., Islam, F., Ali, A., Bhuiyan, A., Ibrahim, M. and Okeyo, A., 2012. Morphometry and performance of Black Bengal goats at the rural community level in Bangladesh. Bangladesh Journal of Animal Science, 41, 83-89.

    Article  Google Scholar 

  • Chu, M.X., Liu, Z.H., Jiao, C.L., He, Y.Q., Fang, L., Ye, S.C., Chen, G.H. and Wang, J.Y., 2007. Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries). Journal of Animal Science, 85, 598-603.

    Article  CAS  Google Scholar 

  • Davis, G.H., 2005. Major genes affecting ovulation rate in sheep. Genetics Selection Evolution, 37, Suppl. S11-23.

    Article  CAS  Google Scholar 

  • Faruque, S., Chowdhury, S., Siddiquee, N. and Afroz, M., 2010. Performance and genetic parameters of economically important traits of Black Bengal goat. Journal of the Bangladesh Agricultural University, 8(1), 67-78.

    Article  Google Scholar 

  • Ghoreishi, H., Fathi-Yosefabad, S., Shayegh, J. and Barzegari, A., 2019. Identification of mutations in BMP15 and GDF9 genes associated with prolificacy of Markhoz goats. Archives Animal Breeding, 62, 565-570.

    Article  Google Scholar 

  • Gootwine, E., 2020. Invited review: opportunities for genetic improvement toward higher prolificacy in sheep. Small Ruminant Research, 186:106090.

    Article  Google Scholar 

  • Gorlov, I.F., Kolosov, Y.A., Shirokova, N.V., Getmantseva, L.V., Slozhenkina, M.I., Mosolova, N.I., Bakoev, N.F., Leonova, M.A., Kolosov, A.Y. and Zlobina, E.Y.J.R.L.S.F.E.N., 2018. GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds. Rendiconti Lincei. Scienze Fisiche e Naturali, 29, 61-66.

    Article  Google Scholar 

  • Hadizadeh, M., Mohammadbadi, M.R., Niazi, A., Esmailizadeh, A. and Gazooei, Y.M., 2014. Search for polymorphism in growth and differentiation factor 9 (GDF9) gene in prolific Beetal and Tali goats (Capra hircus). Journal of Biodiversity and Environmental Sciences, 4, 186-191.

    Google Scholar 

  • Haldar, A., Pal, P., Datta, M., Paul, R., Pal, S.K., Majumdar, D., Biswas, C.K. and Pan, S., 2014. Prolificacy and its relationship with age, body weight, parity, previous litter size and body linear type traits in meat-type goats. Asian-Australasian Journal of Animal Sciences, 27(5), 628-634.

  • Hanrahan, J.P., Gregan, S.M., Mulsant, P., Mullen, M., Davis, G.H., Powell, R. and Galloway, S.M., 2004. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biology of Reproduction. 70, 900-909.

    Article  CAS  Google Scholar 

  • Heikal, H. and El Naby, W., 2017. Genetic improvement of litter size in four goat breeds in Egypt using polymorphism in bone morphogenetic protein 15 gene. Advances in Animal and Veterinary Sciences, 5, 410-415.

    Article  Google Scholar 

  • Husain, S., Amin, M., Islam, A., 1998. Goat production and its breeding strategy in Bangladesh. First National Workshop on Animal Breeding. Bangladesh Agricultural University, Mymensingh, pp. 17-36.

  • Jalbani, M.A., Kaleri, H.A., Baloch, A.H., Bangulzai, N., Bugti, A.G., Ashraf, F., Kaleri, R.R., Jan, M., Bugti, G.A. and Khosa, A.N., 2017. Study of BMP15 gene polymorphisim in Lehri goat breed of Balochistan. Journal of Applied Environmental and Biological Sciences, 7, 84-89.

    Google Scholar 

  • Javanmard, A., Azadzadeh, N. and Esmailizadeh, A.K., 2011. Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds. Veterinary Research Communications, 35, 157-167.

    Article  Google Scholar 

  • Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K.J.M.b., 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35, 1547-1549.

  • Lassoued, N., Benkhlil, Z., Woloszyn, F., Rejeb, A., Aouina, M., Rekik, M., Fabre, S. and Bedhiaf-Romdhani, S., 2017. FecXBara novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine sheep. BMC Genetics, 18, 43.

    Article  Google Scholar 

  • Maitra, A., Sharma, R., Ahlawat, S., Borana, K. and Tantia, M.S., 2016. Fecundity gene SNPs as informative markers for assessment of Indian goat genetic architecture. Indian Journal of Animal Research, 50, 349-356.

    Google Scholar 

  • Mather, K., **ks, J.L., 1977. Introduction to Biometrical Genetics, Chapman and Hall London.

  • Miah, G., Das, A., Bilkis, T., Momin, M.M., Uddin, M.A., Alim, M.A., Mahmud, M.S. and Miazi, O.F., 2016. Comparative study on productive and reproductive traits of Black Bengal and Jamnapari goats under semi-intensive condition. Scientific Research Journal, 4, 1-6.

    Google Scholar 

  • Mishra, C., Rout, M., Mishra, S.P., Sahoo, S.S., Nayak, G. and Patra, R.C., 2017. Genetic polymorphism of prolific genes in goat—a brief review. Exploratory Animal and Medical Research, 7, 132-141.

    Google Scholar 

  • NLDP .2007. National Livestock Development Policy, Department of Livestock Services, Ministry of Fisheries and Livestock, Government of the People’s Republic of Bangladesh.

  • Otsuka, F., McTavish, K.J. and Shimasaki, S., 2011. Integral role of GDF-9 and BMP-15 in ovarian function. Molecular Reproduction and Development, 78, 9-21.

    Article  CAS  Google Scholar 

  • Paul, R., Rahman, A., Debnath, S. and Khandoker, M., 2014. Evaluation of productive and reproductive performance of Black Bengal goat. Bangladesh Journal Animal Science, 43, 104-111.

    Article  Google Scholar 

  • Polley, S., De, S., Batabyal, S., Kaushik, R., Yadav, P., Arora, J.S., Chattopadhyay, S., Pan, S., Brahma, B., Datta, T.K. and Goswami, S.L., 2009. Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat. Small Ruminant Research, 85, 122-129.

    Article  Google Scholar 

  • Shi, Y.Y. and He, L., 2005. SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Research, 15(2), 97-98.

    Article  CAS  Google Scholar 

  • Shokrollahi, B. and Morammazi, S., 2018. Polymorphism of GDF 9 and BMPR 1B genes and their association with litter size in Markhoz goats. Reproduction in Domestic Animals, 53, 971-978.

    Article  CAS  Google Scholar 

  • Vacca, G.M., Dhaouadi, A., Rekik, M., Carcangiu, V., Pazzola, M. and Dettori, M.L., 2010. Prolificacy genotypes at BMPR 1B, BMP15 and GDF9 genes in North African sheep breeds. Small Ruminant Research, 88, 67-71.

    Article  Google Scholar 

  • Wang, X., Yang, Q., Wang, K., Zhang, S., Pan, C., Chen, H., Qu, L., Yan, H. and Lan, X., 2017. A novel 12-bp indel polymorphism within the GDF9 gene is significantly associated with litter size and growth traits in goats. Animal Genetics, 48, 735.

    Article  CAS  Google Scholar 

  • Wang, X., Yang, Q., Zhang, S., Zhang, X., Pan, C., Chen, H., Zhu, H. and Lan, X., 2019. Genetic effects of single nucleotide polymorphisms in the goat GDF9 gene on prolificacy: true or false positive? Animals, 9, 886.

    Article  CAS  Google Scholar 

  • Williams, S.C., 2016. News feature: genetic mutations you want. Proceedings of the National Academy of Sciences of the United States of America, 113, 2554-2557.

    Article  CAS  Google Scholar 

  • Yue, C., Bai, W.L., Zheng, Y.Y., Hui, T.Y., Sun, J.M., Guo, D., Guo, S.L. and Wang, Z.Y.J.A.b., 2019. Correlation analysis of candidate gene SNP for high yield in Liaoning cashmere goats with litter size and cashmere performance. Animal Biotechnology, 19, 1-8.

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Acknowledgements

The study was supported financially by a grant from the University Grants Commission, Bangladesh under a project entitled “Screening genetic markers in fecundity: a savings account for marker-assisted selection in Black Bengal goat”.

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Authors and Affiliations

  1. Department of Genetics and Animal Breeding, Chattogram Veterinary and Animal Sciences University, Zakir Hossain Road, Khulshi, Chattogram, 4225, Bangladesh

    Ashutosh Das, Mishuk Shaha & Omar Faruk Miazi

  2. Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University, Khulshi, Chattogram, 4225, Bangladesh

    Mukta Das Gupta & Avijit Dutta

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  1. Ashutosh Das
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  2. Mishuk Shaha
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  3. Mukta Das Gupta
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  5. Omar Faruk Miazi
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Contributions

A. Das conceived this project. M. Shaha performed sample collection. M. Shaha and A. Dutta extracted DNA and performed PCR. A. Das and M. Das Gupta performed data curation and formal data analysis. A. Das and O. F. Miazi involved in funding acquisition, project administration and supervision. A. Das drafted and finalized the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ashutosh Das.

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Das, A., Shaha, M., Gupta, M.D. et al. Polymorphism of fecundity genes (BMP15 and GDF9) and their association with litter size in Bangladeshi prolific Black Bengal goat. Trop Anim Health Prod 53, 230 (2021). https://doi.org/10.1007/s11250-021-02679-2

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