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Dimethyl tyrosine conjugated peptide prevents oxidative damage and death of triticale and wheat microspores

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Abstract

Functional damage of mitochondria and chloroplasts under stress contributes to reactive oxygen species (ROS) induced cell death. A high percentage of cell mortality during microspore culture is a limiting factor in haploid and doubled haploid plant production. In the present study, we studied the contribution of dimethyl tyrosine (DMT) conjugated short peptides to microspore embryogenesis. These DMT-peptides, which are known to translocate to subcellular targets and scavenge ROS, mitigate the effect oxidative stress plays on microspore viability and embryogenesis. The number of viable microspores was significantly higher in the presence of SS-31 and caspase-3-inhibitor (Ac-DEVD-CHO). In particular, the total number of green plant regeneration was increased by 42 % in the presence of SS-02, and by 55 % in the presence of SS-31, in triticale. Conversely, lower caspase-3-like activities were observed in the presence of SS-31 and Ac-DEVD-CHO, and intracellular ROS was reduced in the presence of SS-31, supporting the involvement of SS-31 in reducing microspore cell death by mitigating ROS and caspase-3-like activity. This study further supports the concept that antioxidant conjugated peptides offer a useful strategy for reducing ROS in plant cells.

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Acknowledgments

We thank Grant Duke for excellent technical assistance for confocal fluorescent microscopic studies and Eric Amundsen for excellent technical assistance for IMC and flow cytometer studies. The research reported in this paper was supported by Western Grains Research Foundation. We thank Ravinder K Goyal and Jordan Pepper for providing an English proof read.

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  1. Cereal Biotechnology, Agriculture and Agri-Food Canada, 5403 - 1 Avenue South, Lethbridge, AB, T1J 4B1, Canada

    Rakesh Kumar Sinha & François Eudes

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  1. Rakesh Kumar Sinha
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Correspondence to François Eudes.

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Sinha, R.K., Eudes, F. Dimethyl tyrosine conjugated peptide prevents oxidative damage and death of triticale and wheat microspores. Plant Cell Tiss Organ Cult 122, 227–237 (2015). https://doi.org/10.1007/s11240-015-0763-x

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  • DOI: https://doi.org/10.1007/s11240-015-0763-x

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