Abstract
Somatic embryos were induced from in vitro germinating seed-hypocotyls of Catharanthus roseus. The process of embryogenesis has been categorized into a few distinct stages (induction, proliferation, maturation and germination) in which liquid overlaying at varying levels 0 ml (T0), 0.25 ml (T1), 0.5 ml (T2), 0.75 ml (T3) and 1.0 ml (T4) was applied on solid medium. It was found that liquid overlaying improved proliferation; maturation, germination of embryos in C. roseus. In proliferation stage, particularly in T2, torpedo embryo number increased significantly (i.e. 129.6%) as compared to control. Liquid overlaying (T2, T3 and T4) also improved embryo maturation and showed early germination even in maturation medium. It also accelerated normal embryo germination frequency particularly in treatment with T2 and shortened ‘embryo—plantlet’ recovery time. Biochemical analyses revealed more proline, protein and amino acid with increasing level of liquid overlaying as it improved embryo induction, development and faster germination.
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Abbreviations
- ANOVA:
-
Analysis of variance
- BA:
-
N6-Benzyladenine
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- GA3 :
-
Gibberellic acid
- KIN:
-
Kinetin
- NAA:
-
Naphthalene acetic acid
- MS:
-
Murashige and Skoog (1962) medium
- TCA:
-
Trichloroacetic acid
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The first author is thankful to Council of Scientific and Industrial Research (CSIR) for receiving Senior Research Fellowship (SRF). Thanks are also due to Dr. Saumitra Banerjee for his help with this manuscript.
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Siddiqui, Z.H., Mujib, A. & Maqsood, M. Liquid overlaying improves somatic embryogenesis in Catharanthus roseus . Plant Cell Tiss Organ Cult 104, 247–256 (2011). https://doi.org/10.1007/s11240-010-9828-z
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DOI: https://doi.org/10.1007/s11240-010-9828-z