Abstract
Laelia speciosa is an endangered epiphytic orchid. The effects of various media components on germination of L. speciosa were evaluated. Pods were collected at 4, 7, and 9 months following hand-pollination, and seeds were germinated on Murashige and Skoog (MS) media with 30 g l−1 sucrose and five concentrations (0.0, 0.04, 0.22, 0.44, and 2.22 μM) of benzyladenine (BA) under light and dark conditions. Gibberellic acid (GA3; 0.0, 0.29, 1.44, 2.89, 14. 43 and 28.87 μM) with naphthaleneacetic acid (NAA; 0.0, 0.54, 1.34, 2.69, and 5.37 μM) were evaluated for in vitro subcultivation. MS medium with 30 g l−1 sucrose was effective for germination. The effects of BA and light on germination of L. speciosa seeds differed with pod maturity. All mature seeds germinated using 0.44 μM BA and light. The highest frequency of germinated seedlings (60%) was obtained using mature seeds grown on MS medium without BA and under light conditions. For subculture, MS with 30 g l−1 sucrose, 2.69 μM NAA, and 0.29 μM GA3 was effective. Plantlets of 5 cm in length were transplanted to the greenhouse, and a 77.5% of survival rate was obtained. A successful protocol for micropropagation by seed germination will contribute to the development of a sustainable management program for L. speciosa.
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Abbreviations
- BA:
-
Benzyladenine
- GA3 :
-
Gibberellic acid
- KC:
-
Knudson C
- MS:
-
Murashige–Skoog medium
- NAA:
-
Naphthaleneacetic acid
- PLBs:
-
Protocorm-like bodies
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Acknowledgments
The authors thank to J. G. García-Franco and two anonymous reviewers for comments to preliminary drafts, and to A. Valencia and M. E. Sarabia for their technical assistance. This research was supported by Fondo Mexicano para la Conservación de la Naturaleza (FMCN, project A 1-99/130), Consejo Nacional de Ciencia y Tecnología (SEMARNAT-CONACYT, project 2002-C01-0544), Coordinación de la Investigación Científica, Universidad Michoacana de San Nicolás de Hidalgo (CIC). IA-D was sponsored by a doctoral scholarship from CONACYT (Reg. 130249) and DGEP-UNAM (Project 202392).
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Ávila-Díaz, I., Oyama, K., Gómez-Alonso, C. et al. In vitro propagation of the endangered orchid Laelia speciosa . Plant Cell Tiss Organ Cult 99, 335–343 (2009). https://doi.org/10.1007/s11240-009-9609-8
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DOI: https://doi.org/10.1007/s11240-009-9609-8