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Polyamine and nitric oxide levels relate with morphogenetic evolution in somatic embryogenesis of Ocotea catharinensis

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Abstract

In this study we examined the effect of polyamines (PAs) putrescine (Put), spermidine (Spd) and spermine (Spm) on growth, morphology evolution, endogenous PAs levels and nitric oxide (NO) release in Ocotea catharinensis somatic embryo cultures. We observed that Spd and Spm reduced culture growth, permitted embryo morphogenetic evolution from the earliest to last embryo development stages, increased endogenous PAs levels, and induced NO release in O. catharinensis somatic embryos. On the other hand, Put had little effect on these parameters. Spd and Spm could successfully be used to promote somatic embryo maturation in O. catharinensis. The results suggest that Spd and Spm have an important role during the growth, development and morphogenetic evolution of somatic embryos, through alterations in the endogenous nitric oxide and PAs metabolism in this species.

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Abbreviations

NO:

Nitric oxide

PAs:

Polyamines

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

WPM:

Wood plant medium

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Acknowledgements

The authors thank Prof. Dr Ana Maria Viana (CCB/UFSC) for somatic embryos supply and Dr NiNi Tun for the support during NO analysis. This work was carried out with financial support from the State of São Paulo Research Foundation (FAPESP) and the National Council for Scientific and Technological Development (CNPq) to E.I.S.F., and a grant by the Deutsche Forschungsgemeinschaft (Sche 207/11-1) to G.E.F.S. This research was supported by FAPESP scholarship and a travel grant by the DAAD (Germany) to C.S.C.

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Authors and Affiliations

  1. Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, Sao Paulo, CP 11461, 05422-970, Brazil

    Claudete Santa-Catarina, Vanildo Silveira & Eny Iochevet Segal Floh

  2. Universität Hannover, Institut für Zierpflanzenbau, AG Spezielle Ertragsphysiologie, Herrenhäuser Str. 2, 30419, Hannover, Germany

    Günther F. E. Scherer

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  1. Claudete Santa-Catarina
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  2. Vanildo Silveira
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  3. Günther F. E. Scherer
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  4. Eny Iochevet Segal Floh
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Correspondence to Claudete Santa-Catarina.

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Santa-Catarina, C., Silveira, V., Scherer, G.F.E. et al. Polyamine and nitric oxide levels relate with morphogenetic evolution in somatic embryogenesis of Ocotea catharinensis . Plant Cell Tiss Organ Cult 90, 93–101 (2007). https://doi.org/10.1007/s11240-007-9259-7

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