Abstract
A propamocarb-responsive gene named CsABC19 was isolated from a cucumber cultivar ‘D0351’ using a homologous cloning strategy. The full-length cDNA of CsABC19 was 921 bp with a complete ORF encoding 306 amino acids. Quantitative real-time PCR analysis revealed that CsABC19 was induced in the root, stem, leaf, and fruit by propamocarb and the expression levels of CsABC19 seemed to be different in different tissues. Further functional analysis showed that CsABC19 transgenic Arabidopsis plants appeared better growth performance under propamocarb stress and lower propamocarb residues. Our findings suggest that CsABC19 plays a crucial role in plant responses to propamocarb stress and also provide new clues for the mechanism regulation of the responses to propamocarb stress in cucumber.
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This work was supported by the National Natural Science Foundation of China (31272158).
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Figure S1
The phenotypes of WT seedlings in the presence of PM. (a) Phenotypes of WT seedlings (7-days-old) grown on MS medium supplemented with 0, 1, 2, 4, 6, or 8 mM PM. (b) Effect on cotyledons opening in WT. (c) Effect on cotyledons opening and greening in WT. Significant differences (P<0.05) are indicated with letters (DOCX 3477 kb)
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Meng, Jj., Qin, Zw., Zhou, Xy. et al. An ATP-Binding Cassette Transporter Gene from Cucumis sativus L., CsABC19, Is Involved in Propamocarb Stress in Arabidopsis thaliana . Plant Mol Biol Rep 34, 947–960 (2016). https://doi.org/10.1007/s11105-016-0976-0
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DOI: https://doi.org/10.1007/s11105-016-0976-0