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Analysis of Expressed Sequence Tags (ESTs) Collected from the Inflorescence of Chrysanthemum

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Abstract

The expressed sequence tags (ESTs) described in this report were obtained from the inflorescence of chrysanthemum. A complementary DNA (cDNA) library was constructed from the inflorescence of the anemone-type chrysanthemum ‘Zhongshanzigui’. In total, 7,307 cDNA clones were sequenced, representing 4,563 unique sequences and consisting of 3,567 singletons and 996 contigs. Comparison to the GenBank nonredundant (nr) database revealed 57.2% (2,608/4,563) chrysanthemum sequences with homology to genes of known function of other organisms. Approximately 26.67% (1,217/4,563) of the unigenes were clustered into 23 categories by the clusters of orthologous group analysis: Most of the identified transcripts were genes related to metabolism, subcellular localization, protein biosynthesis, and cell wall structure. The ESTs presented here will be a valuable addition to floral development transcriptional database.

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Abbreviations

cDNA:

Complementary DNA

CDS:

Coding regions

contigs:

Contiguous consensus sequences

COGs:

The clusters of orthologous groups

ESTs:

Expressed sequence tags

nr:

Nonredundant

SNPs:

Single nucleotide polymorphisms

TFs:

Transcription factors

UTR:

Untranslated region

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Acknowledgments

The authors would like to thank Prof. Hiroaki Kodama of Graduate School of Horticulture, Chiba University, Japan, for his advices on cDNA library construction and Bei**g Genomics Institute (BGI) for EST sequencing and assembling. This work was supported by the Program for New Century Excellent Talents in University of Chinese Ministry of Education (Grant No. NCET-06-0489), the National Natural Science Foundation of China (Grant No. 30872064), China Postdoctoral Science Foundation funded project (Grant No. 20070411058), and National Key Technology R&D Program of the Ministry of Science and Technology of the People’s Republic of China (Grant No. 2006BAD01A1806).

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Authors and Affiliations

  1. Key Laboratory of Flower Breeding and Genetics, College of Horticulture, Nan**g Agricultural University, 210095, Nan**g, China

    Sumei Chen, Hengbin Miao, Fadi Chen, Beibei Jiang, Jungang Lu & Weimin Fang

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  1. Sumei Chen
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  2. Hengbin Miao
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  3. Fadi Chen
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  4. Beibei Jiang
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  5. Jungang Lu
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Corresponding author

Correspondence to Fadi Chen.

Additional information

S. Chen and H. Miao contributed equally to this work reported here.

The chrysanthemum EST sequences have been submitted to the DDBJ Nucleotide Sequence Database under accession nos. DK936567–DK943490.

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Chen, S., Miao, H., Chen, F. et al. Analysis of Expressed Sequence Tags (ESTs) Collected from the Inflorescence of Chrysanthemum. Plant Mol Biol Rep 27, 503–510 (2009). https://doi.org/10.1007/s11105-009-0103-6

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