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Genetic Analysis of Porphyra yezoensis Using Microsatellite Markers

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Abstract

Microsatellites are repetitive genomic elements that show high levels of variation and therefore are useful tools for studying genetic polymorphism and constructing genetic linkage maps of eukaryotic organisms. Porphyra yezoensis Ueda is an economically important seaweed that is being targeted for genetic improvement using marker-assisted breeding. Hence, in an attempt to develop microsatellite markers for P. yezoensis, a microsatellite (or simple sequence repeat)-enriched library was constructed to identify (GA)n and (CA)n motifs. A total of 71 perfect microsatellite clones were identified, of which 30 simple sequence repeat primer pairs were developed. Of these, 24 (80%) amplified polymerase chain reaction products of expected sizes. Twelve primer pairs amplified two to four bands, whereas another 12 primer pairs produced monomorphic banding patterns. Data for 12 loci were analyzed using POPGENE software version 1.32. A total of 29 alleles were produced at 12 loci, with an average of 2.42 alleles (Na) and 1.81 effective alleles (Ne) per locus. These markers were used to analyze the genetic diversity within 11 geographically different lines of P. yezoensis. Overall, these lines were clustered into two divisions with those from close geographic locations clustering together. Further cloning and sequencing of size variant alleles at two microsatellite loci revealed that the variable numbers of motif repeats in different alleles were major sources of polymorphisms.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 30700621), National High Technology Research and Development Program of China (Grant Nos. 2006AA10A402, 2006AA10A413), the Key Project of Chinese Ministry of Education (Grant No. 107070), Program for New Century Excellent Talents in University (Grant No. NCET-06-0596), and Project of National Infrastructure of Natural Resources for Science and Technology (Grant No. 2004DKA30470-016).

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Fanna Kong, Yunxiang Mao, Hui Yang & Li Wang

  2. Key Laboratory of Marine Genetics and Gene Resource Exploitation of Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Fanna Kong, Yunxiang Mao, Hui Yang & Li Wang

  3. The Affiliated Hospital of Medical College, Qingdao University, Qingdao, 266003, China

    Haijun Qu

  4. College of Aqua-Life Science and Technology, Shanghai Fisheries University, Shanghai, 200090, China

    **nghong Yan

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  1. Fanna Kong
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Correspondence to Yunxiang Mao.

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Kong, F., Mao, Y., Yang, H. et al. Genetic Analysis of Porphyra yezoensis Using Microsatellite Markers. Plant Mol Biol Rep 27, 496–502 (2009). https://doi.org/10.1007/s11105-009-0101-8

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