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GUSP1 and GUSP2, Two Drought-Responsive Genes in Gossypium arboreum Have Homology to Universal Stress Proteins

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Abstract

Two closely related genes GUSP1 and GUSP2, within the universal stress protein (USP) family, were identified and cloned from water-stressed leaves of Gossypium arboreum. GUSP1 and GUSP2 genes code for proteins with predicted molecular weights of 18.2 and 19.1 kDa, respectively. Sequence analysis showed that GUSP1 and GUSP2 are highly similar to the bacterial MJ0577-type of adenosine-triphosphate-binding Usp proteins, which have been proposed to function as a molecular switch. Nucleotide sequences of these two genes showed 81% sequence similarity while their encoded proteins share 75% amino acid homology. Both proteins have high percentages of similarity (17% to 61%) to the USPs from a variety of bacteria and plants. Real-time polymerase chain reaction expression analysis revealed a high level of GUSP gene expression in leaves, roots, and stems exclusively in plants following water stress. The highest levels of drought-inducible expression were found in the leaves. A progressive decrease in expression was observed in the stem and roots compared to very low expression in control tissues.

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Acknowledgements

The authors gratefully acknowledge the grants from Higher Education Commission and Ministry of Education, Government of Pakistan.

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Authors and Affiliations

  1. Center of Excellence in Molecular Biology, University of the Punjab, 87-Canal Bank Road, Thokar Niaz Baig, Lahore, 53700, Pakistan

    Asma Maqbool, Muzna Zahur, Tayyab Husnain & Shiekh Riazuddin

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  1. Asma Maqbool
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  2. Muzna Zahur
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  3. Tayyab Husnain
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  4. Shiekh Riazuddin
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Correspondence to Tayyab Husnain.

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Maqbool, A., Zahur, M., Husnain, T. et al. GUSP1 and GUSP2, Two Drought-Responsive Genes in Gossypium arboreum Have Homology to Universal Stress Proteins. Plant Mol Biol Rep 27, 109–114 (2009). https://doi.org/10.1007/s11105-008-0049-0

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