Abstract
Purpose
Applying excessive N is a common strategy in tea plantations. Fungal and bacterial responses to N fertiliser addition in tea plantations, especially their relationship with tea growth, quality, and soil microbiome composition, remain unclear.
Methods
We performed a field experiment using different N fertiliser application rates for 5 years (2016‒2020) in a tea-producing region of China.
Results
The N application rate of 360 kg ha−1 y−1 achieved the highest tea yield and quality. Bacterial diversity and community responses to N addition were more apparent than fungal diversity and community responses. Partial least square path modelling suggested that N addition directly affected the diversity and communities of bacteria and fungi and indirectly affected bacterial community and fungal diversity by altering soil contents. N fertiliser application contributed to tea growth but did not maintain high microbial diversity.
Conclusion
Fungal alpha and beta diversity had a greater effect on tea yield and quality than bacterial diversity; therefore, more attention should be given to fungi such as ligninolytic and cellulolytic taxa, which play a stable role in nutrient cycling and organic matter decomposition in tea plantations, favouring tea growth in the long term.
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Abbreviations
- AA:
-
Amino acid
- AK:
-
NH4OAc-K
- AN:
-
Alkali-hydrolysable N
- ANOVA:
-
One-way analysis of variance
- AP:
-
Available phosphorus
- NCM:
-
Neutral community model;
- NMDS:
-
Non-metric multidimensional scaling
- OTU:
-
Operational taxonomic unit
- PLS-PM:
-
Partial least squares path modelling
- RDA:
-
Redundancy analysis
- SOC:
-
Soil organic carbon
- TN:
-
Total nitrogen
References
Bahram M, Hildebrand F, Forslund SK, Anderson JL, Soudzilovskaia NA, Bodegom PM, Bengtsson-Palme J, Anslan S, Coelho LP, Harend H, Huerta-Cepas J, Medema MH, Maltz MR, Mundra S, Olsson PA, Pent M, Põlme S, Sunagawa S, Ryberg M, Tedersoo L, Bork P (2018) Structure and function of the global topsoil microbiome. Nature 560:233–237. https://doi.org/10.1038/s41586-018-0386-6
Ballhausen MB, de Boer W (2016) The sapro-rhizosphere: Carbon flow from saprotrophic fungi into fungus-feeding bacteria. Soil Biol Biochem 102:14–17. https://doi.org/10.1016/j.soilbio.2016.06.014
Bao S (2000) Soil and Agricultural Chemistry Analysis. China Agricultural Press, Bei**g
Beales N (2004) Adaptation of microorganisms to cold temperatures, weak acid preservatives, low pH, and osmotic stress: A review. Compr Rev Food Sci Food Safety 3:1–20. https://doi.org/10.1111/j.1541-4337.2004.tb00057.x
Burns AR, Stephens WZ, Stagaman K, Wong S, Rawls JF, Guillemin K, Bohannan BJM (2016) Contribution of neutral processes to the assembly of gut microbial communities in the zebrafish over host development. ISME J 10:655–664. https://doi.org/10.1038/ismej.2015.142
Chao A (1984) Nonparametric estimation of the number of classes in a population. Scand J Stat 11:265–270. https://www.jstor.org/stable/4615964
Chen CF, Hu CY, Liou ML, Wu CC, Su YS, Liu CJ (2014) Application of Low-Phosphorous Fertilizers on Tea Plantations as a Novel Best Management Practice. Sustainability 6:6985–6997. https://doi.org/10.3390/su6106985
Chen J, Wang PF, Wang C, Wang X, Miao LZ, Liu S, Yuan QS, Sun SH (2020) Fungal community demonstrates stronger dispersal limitation and less network connectivity than bacterial community in sediments along a large river. Environ Microbiol 22:832–849. https://doi.org/10.1111/1462-2920.14795
Chen QY, Liu ZJ, Zhou JB, Xu XP, Zhu YJ (2021) Long-term straw mulching with nitrogen fertilization increases nutrient and microbial determinants of soil quality in a maize-wheat rotation on China’s Loess Plateau. Sci Total Environ 775:12. https://doi.org/10.1016/j.scitotenv.2021.145930
Chen WD, Ren KX, Isabwe A, Chen HH, Liu M, Yang J (2019) Stochastic processes shape microeukaryotic community assembly in a subtropical river across wet and dry seasons. Microbiome 7:2. https://doi.org/10.1186/s40168-019-0749-8
Dai ZM, Su WQ, Chen HH, Barberán A, Zhao HC, Yu MJ, Yu L, Brookes PC, Schadt CW, Chang SX, Xu JM (2018) Long-term nitrogen fertilization decreases bacterial diversity and favors the growth of Actinobacteria and Proteobacteria in agro-ecosystems across the globe. Glob Change Biol 24:3452–3461. https://doi.org/10.1111/gcb.14163
de Boer W, Folman LB, Summerbell RC, Boddy L (2005) Living in a fungal world: impact of fungi on soil bacterial niche development. FEMS Microbiol Rev 29:795–811. https://doi.org/10.1016/j.femsre.2004.11.005
de Vries FT, Griffiths RI, Bailey M, Craig H, Girlanda M, Gweon HS, Hallin S, Kaisermann A, Keith AM, Kretzschmar M, Lemanceau P, Lumini E, Mason KE, Oliver A, Ostle N, Prosser JI, Thion C, Thomson B, Bardgett RD (2018) Soil bacterial networks are less stable under drought than fungal networks. Nat Commun 9:3033. https://doi.org/10.1038/s41467-018-05516-7
Entwistle EM, Zak DR, Argiroff WA (2018) Anthropogenic N deposition increases soil C storage by reducing the relative abundance of lignolytic fungi. Ecol Monogr 88:225–244. https://doi.org/10.1002/ecm.1288
Fan DM, Fan K, Yu CP, Lu YT, Wang XC (2017) Tea polyphenols dominate the short-term tea (Camellia sinensis) leaf litter decomposition. J Zhejiang Univ Sci B 18:99–108. https://doi.org/10.1631/jzus.B1600044
Fan K, Wang M, Gao YY, Ning QY, Shi YZ (2019) Transcriptomic and ionomic analysis provides new insight into the beneficial effect of Al on tea roots’ growth and nutrient uptake. Plant Cell Rep 38:715–729. https://doi.org/10.1007/s00299-019-02401-5
FAO (2019) (Food and Agriculture Organization of the United Nations). Production FAOSTAT. Available via http://faostat3.fao.org
Fierer N, Bradford MA, Jackson RB (2007) Toward an ecological classification of soil bacteria. Ecology 88:1354–1364. https://doi.org/10.1890/05-1839
Fierer N, Lauber CL, Ramirez KS, Zaneveld J, Bradford MA, Knight R (2012) Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients. ISME J 6:1007–1017. https://doi.org/10.1038/ismej.2011.159
Geisseler D, Scow KM (2014) Long-term effects of mineral fertilizers on soil microorganisms - A review. Soil Biol Biochem 75:54–63. https://doi.org/10.1016/j.soilbio.2014.03.023
Glassman SI, Wang IJ, Brun TD (2017) Environmental filtering by pH and soil nutrients drives community assembly in fungi at fine spatial scales. Mol Ecol 26:6960–6973. https://doi.org/10.1111/mec.14414
Guo JH, Liu XJ, Zhang Y, Shen JL, Han WX, Zhang WF, Christi P, Goulding KW, Vitousek PM, Zhang FS (2010) Significant acidification in major Chinese croplands. Science 327:1008–1010. https://doi.org/10.1126/science.1182570
Hogberg MN, Hogberg P, Myrold DD (2007) Is microbial community composition in boreal forest soils determined by pH, C-to-N ratio, the trees, or all three? Oecologia 150:590–601. https://doi.org/10.1007/s00442-006-0562-5
Hu XF, Chen FS, Wine ML, Fang XM (2017) Increasing acidity of rain in subtropical tea plantation alters aluminum and nutrient distributions at the root-soil interface and in plant tissues. Plant Soil 417:261–274. https://doi.org/10.1007/s11104-017-3256-3
Huang J, Xu CC, Ridout BG, Wang XC, Ren PA (2017) Nitrogen and phosphorus losses and eutrophication potential associated with fertilizer application to cropland in China. J Clean Prod 159:171–179. https://doi.org/10.1016/j.jclepro.2017.05.008
Ji L, Ni K, Ma L, Chen Z, Zhao Y, Ruan J, Guo S (2018) Effect of different fertilizer regimes on the fungal community of acidic tea-garden soil. Acta Ecol Sin 38:8158–8166. https://doi.org/10.5846/stxb201710151847
Ji L, Wu Z, You Z, Yi X, Ni K, Guo S, Ruan J (2018) Effects of organic substitution for synthetic N fertilizer on soil bacterial diversity and community composition: A 10-year field trial in a tea plantation. Agric Ecosyst Environ 268:124–132. https://doi.org/10.1016/j.agee.2018.09.008
Ji LF, Ni K, Wu ZD, Zhang JW, Yi XY, Yang XD, Ling N, You ZM, Guo SW, Ruan JY (2020) Effect of organic substitution rates on soil quality and fungal community composition in a tea plantation with long-term fertilization. Biol Fertil Soils 56:633–646. https://doi.org/10.1007/s00374-020-01439-y
Jiao S, Peng Z, Qi A, Gao J, Wei G (2021) Linking bacterial-fungal relationships to microbial diversity and soil nutrient cycling. MSystems 6:1052–20. https://doi.org/10.1128/mSystems.01052-20
Jiao S, Zhang B, Zhang G, Chen W, Wei G (2021) Stochastic community assembly decreases soil fungal richness in arid ecosystems. Mol Ecol 30:4338–4348. https://doi.org/10.1111/mec.16047
Kuypers MMM, Marchant HK, Kartal B (2018) The microbial nitrogen-cycling network. Nat Rev Microbiol 16:263–276. https://doi.org/10.1038/nrmicro.2018.9
Lammel DR, Barth G, Ovaskainen O, Cruz LM, Zanatta JA, Ryo M, de Souza EM, Pedrosa FO (2018) Direct and indirect effects of a pH gradient bring insights into the mechanisms driving prokaryotic community structures. Microbiome 6:13. https://doi.org/10.1186/s40168-018-0482-8
Leff JW, Jones SE, Prober SM, Barberán A, Borer ET, Firn JL, Harpole WS, Hobbie SE, Hofmockel KS, Knops MH, McCulley RL, La Pierre K, Risch AC, Seabloom EW, Schütz M, Steenbock C, Stevens CJ, Fierer N (2015) Consistent responses of soil microbial communities to elevated nutrient inputs in grasslands across the globe. Proc Natl Acad Sci USA 112:10967–10972. https://doi.org/10.1073/pnas.1508382112
Li J, Sang CP, Yang JY, Qu LR, **a ZW, Sun H, Jiang P, Wang XG, He HB, Wang C (2021) Stoichiometric imbalance and microbial community regulate microbial elements use efficiencies under nitrogen addition. Soil Biol Biochem 156:108207. https://doi.org/10.1016/j.soilbio.2021.108207
Li JH, Zhang R, Cheng BH, Ye LF, Li WJ, Shi XM (2021) Effects of nitrogen and phosphorus additions on decomposition and accumulation of soil organic carbon in alpine meadows on the Tibetan Plateau. Land Degrad Dev 32:1467–1477. https://doi.org/10.1002/ldr.3792
Li X, Li MH, Deng WW, Ahammed GJ, Wei JP, Yan P, Zhang LP, Fu JY, Han WY (2020) Exogenous melatonin improves tea quality under moderate high temperatures by increasing epigallocatechin-3-gallate and theanine biosynthesis in Camellia sinensis L. J Plant Physiol 253:153273. https://doi.org/10.1016/j.jplph.2020.153273
Li YL, Tremblay J, Bainard LD, Cade-Menun B, Hamel C (2020) Long-term effects of nitrogen and phosphorus fertilization on soil microbial community structure and function under continuous wheat production. Environ Microbiol 22:1066–1088. https://doi.org/10.1111/1462-2920.14824
Liu WX, Liu LL, Yang X, Deng MF, Wang Z, Wang PD, Yang S, Li P, Peng ZY, Yang L, Jiang L (2021) Long-term nitrogen input alters plant and soil bacterial, but not fungal beta diversity in a semiarid grassland. Glob Change Biol 27:3939–3950. https://doi.org/10.1111/gcb.15681
Louca S, Polz MF, Mazel F, Albright MBN, Huber JA, O’Connor MI, Ackermann M, Hahn AS, Srivastava DS, Crowe SA, Doebeli M, Parfrey LW (2018) Function and functional redundancy in microbial systems. Nat Ecol Evol 2:936–943. https://doi.org/10.1038/s41559-018-0519-1
Lucas RW, Klaminder J, Futter MN, Bishop KH, Egnell G, Laudon H, Hogberg P (2011) A meta-analysis of the effects of nitrogen additions on base cations: Implications for plants, soils, and streams. Ecol Manage 262:95–104. https://doi.org/10.1016/j.foreco.2011.03.018
Ma L, Chen J, Shan Y, Jiang M, Zhang G, Wu L, Ruan J, Lv J, Shi Y, Pan L (2013) Status and suggestions of tea garden fertilization on main green tea-producing counties in Zhejiang Province. J Tea Sci 33:74–84. https://doi.org/10.13305/j.cnki.jts.2013.01.010
Ma Q, Wen Y, Wang D, Sun X, Hill PW, Macdonald A, Chadwick DR, Wu L, Jones DL (2020) Farmyard manure applications stimulate soil carbon and nitrogen cycling by boosting microbial biomass rather than changing its community composition. Soil Biol Biochem 144:107760. https://doi.org/10.1016/j.soilbio.2020.107760
Ma L, Yang X, Shi Y, Yi X, Ji L, Cheng Y, Ni K, Ruan J (2021) Response of tea yield, quality and soil bacterial characteristics to long-term nitrogen fertilization in an eleven-year field experiment. Appl Soil Ecol 166:103976
Ma Q, Kuzyakov Y, Pan W, Tang S, Chadwick DR, Wen Y, Hill PW, Macdonald A, Ge T, Si L, Wu L, Jones DL (2021) Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling. ISME J 15:3148–3158. https://doi.org/10.1038/s41396-021-00999-7
Musial C, Kuban-Jankowska A, Gorska-Ponikowska M (2020) Beneficial properties of green tea catechins. Int J of Mol Sci 21:1744. https://doi.org/10.3390/ijms21051744
Naylor D, Sadler N, Bhattacharjee A, Graham EB, Anderton CR, McClure R, Lipton M, Hofmockel KS, Jansson JK (2020) Soil microbiomes under climate change and implications for carbon cycling. In: Gadgil A, Tomich TP (eds.) Annual Review of Environment and Resources, Vol 45. Annual Reviews, Palo Alto, pp 29–59.
Ni K, Liao W, Yi X, Niu S, Ma L, Shi Y, Zhang Q, Liu M, Ruan J (2019) Fertilization status and reduction potential in tea gardens of China. J Plant Nutr and Fertil 25:421–432
Qiao CL, Xu B, Han YT, Wang J, Wang X, Liu LL, Liu WX, Wan SQ, Tan H, Liu YZ, Zhao XM (2018) Synthetic nitrogen fertilizers alter the soil chemistry, production and quality of tea. A meta-analysis. Agron Sustain Dev 38:10. https://doi.org/10.1007/s13593-017-0485-z
Reay MK, Charteris AF, Jones DL, Evershed RP (2019) 15N-amino sugar stable isotope probing (15N-SIP) to trace the assimilation of fertiliser-N by soil bacterial and fungal communities. Soil Biol Biochem 138. https://doi.org/10.1016/j.soilbio.2019.107599
Rousk J, Baath E, Brookes PC, Lauber CL, Lozupone C, Caporaso JG, Knight R, Fierer N (2010) Soil bacterial and fungal communities across a pH gradient in an arable soil. ISME J 4:1340–1351. https://doi.org/10.1038/ismej.2010.58
Ruan J, Gerendas J, Haerdter R, Sattelmacher B (2007) Effect of root zone pH and form and concentration of nitrogen on accumulation of quality-related components in green tea. J Sci Food Agric 87:1505–1516. https://doi.org/10.1002/jsfa.2875
Ruan J, Haerdter R, Gerendas J (2010) Impact of nitrogen supply on carbon/nitrogen allocation: a case study on amino acids and catechins in green tea [Camellia sinensis (L.) O. Kuntze] plants. Plant Biol 12:724–734. https://doi.org/10.1111/j.1438-8677.2009.00288.x
Ruan J, Ma L, Shi Y (2013) Potassium management in tea plantations: Its uptake by field plants, status in soils, and efficacy on yields and quality of teas in China. J Plant Nutr Soil Sci 176:450–459. https://doi.org/10.1002/jpln.201200175
Ruan J, Ma L, Yi X, Shi Y, Ni K, Liu M, Zhang Q (2020) Integrated nutrient management in tea plantation to reduce chemical fertilizer and increase nutrient use efficiency. J Tea Sci 40:85–95. https://doi.org/10.13305/j.cnki.jts.20200117.003
Saleem M, Hu J, Jousset A (2019) More than the sum of its parts: Microbiome biodiversity as a driver of plant growth and soil health. In: Futuyma DJ (Ed) Annual Review of Ecology, Evolution, and Systematics, Vol 50. Annual Reviews, Palo Alto, pp 145–168.
Schmidt SK, Nemergut DR, Darcy JL, Lynch R (2014) Do bacterial and fungal communities assemble differently during primary succession? Mol Ecol 23:254–258. https://doi.org/10.1111/mec.12589
Schneider T, Keiblinger KM, Schmid E, Sterflinger-Gleixner K, Ellersdorfer G, Roschitzki B, Richter A, Eberl L, Zechmeister-Boltenstern S, Riedel K (2012) Who is who in litter decomposition? Metaproteomics reveals major microbial players and their biogeochemical functions. ISME J 6:1749–1762. https://doi.org/10.1038/ismej.2012.11
Shade A (2017) Diversity is the question, not the answer. ISME J 11:1–6. https://doi.org/10.1038/ismej.2016.118
Shannon CE (1948) A mathematical theory of communication. Bell Sys Tech J 27:379–423. https://doi.org/10.1002/j.1538-7305.1948.tb01338.x
Shi L, Zhang H, Liu T, Mao P, Zhang W, Shao Y, Fu S (2018) An increase in precipitation exacerbates negative effects of nitrogen deposition on soil cations and soil microbial communities in a temperate forest. Environ Pollut 235:293–301. https://doi.org/10.1016/j.envpol.2017.12.083
Shi Y, Li YT, **ang XJ, Sun RB, Yang T, He D, Zhang KP, Ni YY, Zhu YG, Adams JM, Chu HY (2018) Spatial scale affects the relative role of stochasticity versus determinism in soil bacterial communities in wheat fields across the North China Plain. Microbiome 6:12. https://doi.org/10.1186/s40168-018-0409-4
Shu WS, Zhang ZQ, Lan CY, Wong MH (2003) Fluoride and aluminium concentrations of tea plants and tea products from Sichuan Province, PR China. Chemosphere 52:1475–1482. https://doi.org/10.1016/S0045-6535(03)00485-5
Sloan WT, Lunn M, Woodcock S, Head IM, Nee S, Curtis TP (2006) Quantifying the roles of immigration and chance in sha** prokaryote community structure. Environ Microbiol 8:732–740. https://doi.org/10.1111/j.1462-2920.2005.00956.x
Stegen JC, Lin XJ, Fredrickson JK, Chen XY, Kennedy DW, Murray CJ, Rockhold ML, Konopka A (2013) Quantifying community assembly processes and identifying features that impose them. ISME J 7:2069–2079. https://doi.org/10.1038/ismej.2013.93
Sun LL, Liu Y, Wu LQ, Liao H (2019) Comprehensive analysis revealed the close relationship between N/P/K status and secondary metabolites in tea leaves. ACS Omega 4:176–184. https://doi.org/10.1021/acsomega.8b02611
Tang S, Liu YL, Zheng N, Li Y, Ma QX, **ao H, Zhou X, Xu XP, Jiang TM, He P, Wu LH (2020) Temporal variation in nutrient requirements of tea (Camellia sinensis) in China based on QUEFTS analysis. Sci Rep 10. https://doi.org/10.1038/s41598-020-57809-x
Tang S, Ma Q, Luo J, **e Y, Hashmi MLUR, Pan W, Zheng N, Liu M, Wu L (2021) The inhibition effect of tea polyphenols on soil nitrification is greater than denitrification in tea garden soil. Sci Total Environ 778:146328–146328. https://doi.org/10.1016/j.scitotenv.2021.146328
Tang S, Zheng N, Ma Q, Zhou J, Sun T, Zhang X, Wu L (2021) Applying nutrient expert system for rational fertilisation to tea (Camellia sinensis) reduces environmental risks and increases economic benefits. J Clean Prod 305:127197. https://doi.org/10.1016/j.jclepro.2021.127197
Tian DS, Niu SL (2015) A global analysis of soil acidification caused by nitrogen addition. Environ Res Lett 10:024019. https://doi.org/10.1088/1748-9326/10/2/024019
Tripathi BM, Stegen JC, Kim M, Dong K, Adams JM, Lee YK (2018) Soil pH mediates the balance between stochastic and deterministic assembly of bacteria. ISME J 12:1072–1083. https://doi.org/10.1038/s41396-018-0082-4
Wang C, Liu DW, Bai E (2018) Decreasing soil microbial diversity is associated with decreasing microbial biomass under nitrogen addition. Soil Biol Biochem 120:126–133. https://doi.org/10.1016/j.soilbio.2018.02.003
Wang JY, Chadwick DR, Cheng Y, Yan XY (2018) Global analysis of agricultural soil denitrification in response to fertilizer nitrogen. Sci Total Environ 616:908–917. https://doi.org/10.1016/j.scitotenv.2017.10.229
Wang ZT, Geng YB, Liang T (2020) Optimization of reduced chemical fertilizer use in tea gardens based on the assessment of related environmental and economic benefits. Sci Total Environ 713:136439. https://doi.org/10.1016/j.scitotenv.2019.136439
Widdig M, Heintz-Buschart A, Schleuss PM, Guhr A, Borer ET, Seabloom EW, Spohn M (2020) Effects of nitrogen and phosphorus addition on microbial community composition and element cycling in a grassland soil. Soil Biol Biochem 151:108041. https://doi.org/10.1016/j.soilbio.2020.108041
**ong W, Li R, Ren Y, Liu C, Zhao QY, Wu HS, Jousset A, Shen QR (2017) Distinct roles for soil fungal and bacterial communities associated with the suppression of vanilla Fusarium wilt disease. Soil Biol Biochem 107:198–207. https://doi.org/10.1016/j.soilbio.2017.01.010
Yan P, Shen C, Fan L, Li X, Zhang L, Zhang L, Han W (2018) Tea planting affects soil acidification and nitrogen and phosphorus distribution in soil. Agric Ecosys Environ 254:20–25. https://doi.org/10.1016/j.agee.2017.11.015
Yang X, Ni K, Shi Y, Yi X, Zhang Q, Fang L, Ma L, Ruan J (2018) Effects of long-term nitrogen application on soil acidification and solution chemistry of a tea plantation in China. Agricu Ecosys Environ 252:74–82. https://doi.org/10.1016/j.agee.2017.10.004
Yang XD, Ma LF, Ji LF, Shi YZ, Yi XY, Yang QL, Ni K, Ruan JY (2019) Long-term nitrogen fertilization indirectly affects soil fungi community structure by changing soil and pruned litter in a subtropical tea (Camellia sinensis L.) plantation in China. Plant Soil 444:409–426. https://doi.org/10.1007/s11104-019-04291-8
Yao F, Yang S, Wang ZR, Wang X, Ye J, Wang XG, DeBruyn JM, Feng X, Jiang Y, Li H (2017) Microbial taxa distribution is associated with ecological trophic cascades along an elevation gradient. Front Microbiol 8:2071. https://doi.org/10.3389/fmicb.2017.02071
Zhang T, Chen HYH, Ruan H (2018) Global negative effects of nitrogen deposition on soil microbes. ISME J 12:1817–1825. https://doi.org/10.1038/s41396-018-0096-y
Zhang XM, Johnston ER, Liu W, Li LH, Han XG (2016) Environmental changes affect the assembly of soil bacterial community primarily by mediating stochastic processes. Glob Change Biol 22:198–207. https://doi.org/10.1111/gcb.13080
Zheng Q, Hu YT, Zhang SS, Noll L, Bockle T, Dietrich M, Herbold CW, Eichorst SA, Woebken D, Richter A, Wanek W (2019) Soil multifunctionality is affected by the soil environment and by microbial community composition and diversity. Soil Biol Biochem 136:107521. https://doi.org/10.1016/j.soilbio.2019.107521
Zheng SJ (2010) Crop production on acidic soils: overcoming aluminium toxicity and phosphorus deficiency. Ann Bot 106:183–184. https://doi.org/10.1093/aob/mcq134
Zheng W, Zhao ZY, Lv FL, Wang RZ, Wang ZH, Zhao ZY, Li ZY, Zhai BN (2021) Assembly of abundant and rare bacterial and fungal sub-communities in different soil aggregate sizes in an apple orchard treated with cover crop and fertilizer. Soil Biol Biochem 156:108222. https://doi.org/10.1016/j.soilbio.2021.108222
Zhou JZ, Ning DL (2017) Stochastic community assembly: Does it matter in microbial ecology? Microbiol Molec Biol Rev 81:e00002-17. https://doi.org/10.1128/MMBR.00002-17
Acknowledgements
This work was supported by the National Natural Science Foundation of China (32102488, 32172674), and the National Key Research and Development Program of China (2020YFD1100402).
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This work was supported by the National Key Research and Development Program of China (Grant number [2020YFD1100402]) and the National Natural Science Foundation of China (Grant numbers [31801936] and [31872180]).
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Sheng Tang: Conceptualisation, Methodology, Formal analysis, Writing - Original draft. Lianghuan Wu: Conceptualisation, Methodology, Formal analysis. Qingxu Ma: Conceptualisation, Methodology, Formal analysis, Writing - Original draft. Tong Qi: Conceptualisation, Methodology, Formal analysis, Writing - Original draft. Zhengbo Ma: Investigation. Rui Tang: Investigation. Wankun Pan: Investigation. Haoran Fu: Investigation. **gjie Zhou: Investigation. Meng Xu: Investigation.
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Tang, S., Zhou, J., Pan, W. et al. Impact of N application rate on tea (Camellia sinensis) growth and soil bacterial and fungi communities. Plant Soil 475, 343–359 (2022). https://doi.org/10.1007/s11104-022-05372-x
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DOI: https://doi.org/10.1007/s11104-022-05372-x