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Molecular characterization of GmSTOP1 homologs in soybean under Al and proton stress

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Abstract

Background and aims

The Sensitive to Proton Rhizotoxicity1 (STOP1) transcription factor has been implicated in the regulation of aluminium (Al) stress and proton toxicity for several plant species. This study aimed to characterize STOP1 homologs in soybean.

Method

Five GmSTOP1 homologs were studied by transcriptional expression, subcellular localization and overexpression experiments.

Results

Five GmSTOP1 homologs were nuclear-localized and exhibited transactivation activity. They constitutively expressed throughout the whole soybean plant. Their expressions were increased from 2 h, peaked at 4 h, returned to basal levels for the remaining duration of Al treatment but varied in aptitude and genotype. They were sensitive to pH conditions with various responses. Overexpression of GmSTOP1a in soybean hairy root increased the expression of the malate transporter gene GmALMT1, and decreased Al accumulation under Al stress. Its overexpression also regulated some pH-sensitive genes, including GmSTOP1c and GmCIPK23. Overexpression of GmSTOP1a in Arabidopsis slightly increase its Al resistance, and partially restored the root growth of the atstop1 mutant under Al stress.

Conclusion

GmSTOP1a contributes to both proton and Al resistance and plays a role similar to that of AtSTOP1. The functions of other four GmSTOP1 genes need further clarified.

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Fig. 1: Phylogenetic tree and multiple sequence alignment of GmSTOP1 with known STOP1 orthologous proteins from other plant species.
Fig. 2: Temporal expression of five GmSTOP1 genes in soybean root apices under Al stress.
Fig. 3: Transcriptional expression of five GmSTOP1 genes in response to Cd 2+ , La 3+ , Cu 2+ , and Al 3+ stress in soybean root apices.
Fig. 4: Transcriptional expression of five GmSTOP1 genes in response to different pH conditions.
Fig. 5: Transcriptional expression of five GmSTOP1 genes in soybean different root segments under Al stress.
Fig. 6: Spatial expression of five GmSTOP1-like genes in soybean.
Fig. 7: Subcellular localization of STOP1 and homologous proteins.
Fig. 8: Transactivation activity of GmSTOP1-like protein.
Fig. 9: The transcriptional expression in GmSTOP1a-OE hair roots under Al stress.
Fig. 10: The transcriptional expression in GmSTOP1a-OE hairy roots in response to different pH conditions.
Fig. 11: Phenotype of Al resistance of Arabidopsis atstop1 mutants complementarily expressing GmSTOP1a under Al stress.
Fig. 12: Phenotype of Al resistance of Arabidopsis atstop1 mutants complementarily expressing GmSTOP1a under different pH conditions.

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Abbreviations

Al:

Aluminum

CaMV:

Cauliflower mosaic virus

STOP1:

Sensitive to Proton Rhizotoxicity1

ART1:

Aluminum resistance transcription factor 1

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Acknowledgements

Financial support was provided by National Natural Science Foundation of China (No. 31372124) and Natural Science Foundation of Jilin Province (20130101084JC).

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Authors and Affiliations

  1. Laboratory of Soil and Plant Molecular Genetics, College of Plant Science, Jilin University, Changchun, 130062, China

    Ying Zhou, ZhenMing Yang, Li Gong, RongKun Liu, HaoRan Sun & JiangFeng You

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  1. Ying Zhou
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  2. ZhenMing Yang
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  3. Li Gong
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  4. RongKun Liu
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Correspondence to JiangFeng You.

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Responsible Editor: Jian Feng Ma.

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Zhou, Y., Yang, Z., Gong, L. et al. Molecular characterization of GmSTOP1 homologs in soybean under Al and proton stress. Plant Soil 427, 213–230 (2018). https://doi.org/10.1007/s11104-018-3645-2

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