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Silicon enhances growth independent of silica deposition in a low-silica rice mutant, lsi1

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Abstract

To examine whether silica bodies are essential for silicon-enhanced growth of rice seedlings, we investigated the response of rice, Oryza sativa L., to silicon treatment. Silicic acid treatment markedly enhanced the SPAD (soil plant analytical development) values of leaf blades and the growth and development of leaves and lateral roots in cvs. Hinohikari and Oochikara, and a low-silicon mutant, lsi1. Combination of ethanol–benzene displacement and staining with crystal violet lactone enabled more detailed histochemical analysis to visualize silica bodies in the epidermis under bright-field microscopy. Supply of silicon induced the development of motor cells and silica bodies in epidermal cells in Hinohikari and Oochikara but not or marginal in lsi1. X-ray analytical microscopy detected silicon specifically in the leaf sheath, the outermost part of the stem, and the leaf blade midrib, suggesting that silicon is distributed to tissues involved in maintaining rigidity of the plant to prevent lodging, rather than being passively deposited in growing tissues. Silicon supplied at high dose accumulated in all rice seedlings and enhanced growth and SPAD values with or without silica body formation. Silicon accumulated in the cell wall may play an important physiological role different from that played by the silica deposited in the motor cell and silica bodies.

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Abbreviations

eV:

Electron voltage

Si:

Silicic acid

SPAD:

Soil and plant analysis development

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Acknowledgments

We are grateful to K. Inoue and Y. Kasuya (Kyushu Univ.) for their careful reading of the English manuscript.

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Authors and Affiliations

  1. Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, 812-8581, Japan

    Mami Isa

  2. Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka, 812-8581, Japan

    Shuqin Bai & Takushi Yokoyama

  3. Research Institute for Bioresources, Okayama University, Kurashiki, 847-0021, Japan

    Jian Feng Ma

  4. Coastal Bioenvironment Center, Saga University, Karatsu, 847-0021, Japan

    Yushi Ishibashi

  5. Department of Plant Resources, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan

    Takashi Yuasa & Mari Iwaya-Inoue

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  1. Mami Isa
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  2. Shuqin Bai
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  3. Takushi Yokoyama
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Correspondence to Takashi Yuasa.

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Responsible Editor: Yong Chao Liang.

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Isa, M., Bai, S., Yokoyama, T. et al. Silicon enhances growth independent of silica deposition in a low-silica rice mutant, lsi1 . Plant Soil 331, 361–375 (2010). https://doi.org/10.1007/s11104-009-0258-9

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