Abstract
The bread wheat cultivar Shanrong No.3 (SR3) is a salinity tolerant derivative of an asymmetric somatic hybrid between cultivar **an 177 (JN177) and tall wheatgrass (Thinopyrum ponticum). To reveal some of the mechanisms underlying its elevated abiotic stress tolerance, both SR3 and JN177 were exposed to iso-osmotic NaCl and PEG stress, and the resulting gene expression was analysed using a customized microarray. Some genes associated with stress response proved to be more highly expressed in SR3 than in JN177 in non-stressed conditions. Its unsaturated fatty acid and flavonoid synthesis ability was also enhanced, and its pentose phosphate metabolism was more active than in JN177. These alterations in part accounted for the observed shift in the homeostasis related to reactive oxygen species (ROS). The specific down-regulation of certain ion transporters after a 0.5 h exposure to 340 mM NaCl demonstrated that Na+ uptake occurred rapidly, so that the early phase of salinity stress imposes more than simply an osmotic stress. We discussed the possible effect of the introgression of new genetic materials in wheat genome on stress tolerance.
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Acknowledgments
This work was supported by the National Basic Research 973 Program of China (2009CB118300), the Major Program of the Natural Science Foundation of China (31030053), and the National Transgenic Project (2009ZX08009-082B and 2008ZX08002-002).
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Chun Liu, Shuo Li and Mengcheng Wang contributed equally to the article.
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Liu, C., Li, S., Wang, M. et al. A transcriptomic analysis reveals the nature of salinity tolerance of a wheat introgression line. Plant Mol Biol 78, 159–169 (2012). https://doi.org/10.1007/s11103-011-9854-1
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DOI: https://doi.org/10.1007/s11103-011-9854-1