Abstract
The chenopodiaceae Suaeda salsa L. is a leaf succulent euhalophyte. Shoots of the S. salsa are larger and more succulent when grown in highly saline environments. This increased growth and water uptake has been correlated with a large and specific cellular accumulation of sodium. S. salsa does not have salt glands or salt bladders on its leaves. Thus, this plant must compartmentalize the toxic Na+ in the vacuoles. The ability to compartmentalize sodium may result from a stimulation of the proton pumps that provide the driving force for increased sodium transport into the vacuole. In this work, we isolated the cDNA of the vacuolar membrane proton-translocating inorganic pyrophosphatase (H+-PPase) from S. salsa. The SsVP cDNA contains an uninterrupted open reading frame of 2292 bp, coding for a polypeptide of 764 amino acids. Northern blotting analysis showed that SsVP was induced in salinity treated leaves. The activities of both the V-ATPase and the V-PPase in Arabidopsis overexpressing SsVP-2 is higher markedly than in wild-type plant under 200 mM NaCl and drought stresses. The Overexpression of SsVP can increase salt and drought tolerance of transgenic Arabidopsis.
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Abbreviations
- H+-PPase:
-
H+-pyrophosphatase
- H+-ATPase:
-
H+-adenosine triphosphatase
- RWC:
-
relative water content
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Shanli Guo, Haibo Yib: These authors contributed equally to this work
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Guo, S., Yin, H., Zhang, X. et al. Molecular Cloning and Characterization of a Vacuolar H+-pyrophos-phatase Gene, SsVP, from the Halophyte Suaeda salsa and its Overexpression Increases Salt and Drought Tolerance of Arabidopsis. Plant Mol Biol 60, 41–50 (2006). https://doi.org/10.1007/s11103-005-2417-6
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DOI: https://doi.org/10.1007/s11103-005-2417-6