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The Fed-1 (CAUU)4 element is a 5′ UTR dark-responsive mRNA instability element that functions independently of dark-induced polyribosome dissociation

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Abstract

Darkness rapidly induces a decline in the stability and translation of the pea Ferredoxin-1 (Fed-1) mRNA in transgenic tobacco. Direct half-life measurement showed that mutation of the (CAUU)4 stabilizes Fed-1 mRNA in the dark. (CAUU)1, a feature more common in plant 5′ UTRs than (CAUU)4, confers slight light-responsive mRNA accumulation. At least three but less than 11 CAUU repeats near the 5′ end of the 5′ UTR are required for full light-responsive accumulation. Furthermore, 26 nt of the 5′ UTR, including the (CAUU)4 repeat, is sufficient to confer a significant ∼2.5-fold increase in light-regulated mRNA accumulation when fused to the 5′ end of a heterologous plant mRNA. A mutation of the (CAUU)4 repeat that compromises light-regulated mRNA stability changes in vitro the accessibility of the region to ribonuclease V1 and ribonuclease A suggesting the geometry formed by the repeat may be important for instability. Finally, dark-induced Fed-1 mRNA instability occurs even when most of the mRNA is retained on polyribosomes, and thus is likely an independent event regulated by darkness.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Oklahoma State University, 74078, 246 Noble Research Center, OK , Stillwater, USA

    Sumana Bhat, Angela D. Krueger, Christopher L. Smith, Sharon R. Ford & Marie E. Petracek

  2. College of Life Sciences, Shanghai University, 200436, Shanghai, P.R. China

    Li Tang

  3. Biolex, Raleigh, NC, 27695, USA

    Lynn F. Dickey

  4. Biolex, 27312, 158 cradle st., NC , Pittsboro, USA

    Lynn F. Dickey

Authors
  1. Sumana Bhat
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  2. Li Tang
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  3. Angela D. Krueger
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  4. Christopher L. Smith
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  5. Sharon R. Ford
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  6. Lynn F. Dickey
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  7. Marie E. Petracek
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Correspondence to Marie E. Petracek.

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Bhat, S., Tang, L., Krueger, A.D. et al. The Fed-1 (CAUU)4 element is a 5′ UTR dark-responsive mRNA instability element that functions independently of dark-induced polyribosome dissociation. Plant Mol Biol 56, 761–773 (2004). https://doi.org/10.1007/s11103-004-5109-8

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