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A genetic and structural analysis of the -glycosylation capabilities

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Abstract

The recent draft sequencing of the rice (Oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. In this study, we have not only identified genes putatively encoding enzymes involved in N-glycosylation, but have examined by MALDI-TOF MS the structures of the N-glycans of rice and other monocotyledons (maize, wheat and dates; Zea mays, Triticum aestivum and Phoenix dactylifera); these data show that within the plant kingdom the types of N-glycans found are very similar between monocotyledons, dicotyledons and gymnosperms. Subsequently, we constructed expression vectors for the key enzymes forming plant-typical structures in rice, N-acetylglucosaminyltransferase I (GlcNAc-TI; EC 2.4.1.101), core α1,3-fucosyltransferase (FucTA; EC 2.4.1.214) and β1,2-xylosyltransferase (EC 2.4.2.38) and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form.

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Authors and Affiliations

  1. Department für Chemie, Universität für Bodenkultur, Muthgasse 18, Austria

    Renaud Léonard, Daniel Kolarich, Katharina Paschinger, Friedrich Altmann & Iain Wilson

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  1. Renaud Léonard
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  2. Daniel Kolarich
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  3. Katharina Paschinger
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  4. Friedrich Altmann
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  5. Iain Wilson
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Léonard, R., Kolarich, D., Paschinger, K. et al. A genetic and structural analysis of the -glycosylation capabilities. Plant Mol Biol 55, 631–644 (2004). https://doi.org/10.1007/s11103-004-1558-3

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