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PsbY is required for prevention of photodamage to photosystem II in a PsbM-lacking mutant of Synechocystis sp. PCC 6803

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Photosynthetica

Abstract

The PsbM (3.9 kDa) and PsbY (4.2 kDa) proteins are membrane-spanning, single-helix, subunits associated with the chlorophyll-binding CP47 pre-complex of photosystem II (PSII). Removal of PsbM resulted in accumulation of PSII pre-assembly complexes and impaired electron transfer between the primary (QA) and secondary (QB) plastoquinone electron acceptors of PSII indicating that the QB-binding site and bicarbonate binding to the non-heme iron were altered in this strain. Removal of PsbY alone had only a minor impact on PSII activity but deleting PsbY in the ΔPsbM background led to additional modification of the acceptor side resulting in ΔPsbM:ΔPsbY cells being susceptible to photodamage and this required protein synthesis for recovery. Addition of bicarbonate was able to compensate for the light-induced damage in ΔPsbM:ΔPsbY cells potentially re-occupying the modified bicarbonate-binding site in the ΔPsbM:ΔPsbY strain and complementation of ΔPsbM:ΔPsbY cells with the psbY gene restored the ΔPsbM phenotype.

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Abbreviations

Chl:

chlorophyll

CP43:

43 kDa chlorophyll-binding core antenna protein

CP47:

47 kDa chlorophyll-binding core antenna protein

DCBQ:

2,6-dichloro-1,4-benzoquinone

DCMU:

3,4-dichloro-1,1-dimethyl urea

DMBQ:

2,5-dimethyl-1,4- benzoquinone

F:

fluorescence level

Fm :

maximum fluorescence level

Fo :

initial fluorescence level

HEPES:

4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid

LMW:

lte]ow molecular weight

PCC:

Pasteur Culture Collection

QA :

primary plastoquinone electron acceptor of PSII

QB :

secondary plastoquinone electron acceptor of PS II

RC47:

a PSII pre-assembly complex composed of the reaction center assembly module and the CP47 pre-complex assembly module

S2:

the oxidation state of the oxygen-evolving complex following a single-turnover flash applied to dark-adapted cells

TES:

2-[tris(hydroxymethyl)methyl]amino-1-ethanesulfonic acid

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Authors and Affiliations

  1. Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, 9054, New Zealand

    S. Biswas & J. J. Eaton-Rye

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  1. S. Biswas
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  2. J. J. Eaton-Rye
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Correspondence to J. J. Eaton-Rye.

Additional information

Acknowledgments: S.B. was supported by an Otago University PhD scholarship. We thank Matthew Prouse for construction of the ΔPsbM and ΔPsbY strains.

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Biswas, S., Eaton-Rye, J.J. PsbY is required for prevention of photodamage to photosystem II in a PsbM-lacking mutant of Synechocystis sp. PCC 6803. Photosynthetica 56, 200–209 (2018). https://doi.org/10.1007/s11099-018-0788-6

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  • DOI: https://doi.org/10.1007/s11099-018-0788-6

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