Abstract
Astrocytes are activated by ciliary neurotrophic factor (CNTF) in vivo and in vitro, however, the consequences on the L-type calcium channel (LCC) of neurons are still poorly understood. Therefore, in the present study, whole-cell patch clamp, western-blot and RT-PCR assay were performed to evaluate the effects of CNTF-treated astrocyte conditioned medium (CNTF-ACM) on LCC current (ICa-L) and the expression of Cav1.2 and Cav1.3 in Sprague–Dawley rat cortical neurons. The results revealed that CNTF-ACM enhanced the amplitude of Ica-L and the expression of Cav1.3 significantly, but had no effects on Cav1.2 expression. We also found an increase in the concentration of fibroblast growth factor-2 (FGF-2) in CNTF-ACM by ELISA assay. Taken together, these findings indicate that CNTF induces the release of factors, including FGF-2, from astrocytes, thereby potentiating the activity of LCC in cortical neurons.
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This work was supported by National Key Natural Science Foundation of China (grant numbers: 30230140) and Doctor Point Foundation of Ministry of Education of China (grant number 20040487060).
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**ao**g Wang and Honghua Zheng contributed equally.
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Wang, X., Zheng, H., Liu, C. et al. Ciliary Neurotrophic Factor-treated Astrocyte Conditioned Medium Regulates the L-type Calcium Channel Activity in Rat Cortical Neurons. Neurochem Res 33, 826–832 (2008). https://doi.org/10.1007/s11064-007-9514-7
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DOI: https://doi.org/10.1007/s11064-007-9514-7