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Assessment of Oxidative Damage Induced by Acute Doses of Morphine Sulfate in Postnatal and Adult Rat Brain

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Abstract

The aim of the present study is to evaluate the oxidative damage in rats of different ages. Weaned rats of 25 g and adults of 300 g were used in groups of 6, a single i.p. dose of morphine sulfate of 3, 6 or 12 mg/kg was administered. All animals were sacrificed to measure GSH and 5-HT levels in brain by liquid chromatography, as well as Na+, K+-ATPase and total ATPase enzymatic activity. 5-HT levels decreased significantly (p<0.05) in adult animals that received 3 and 6 mg morphine. Na+, K+-ATPase activity increased significantly (p<0.05) in all groups of weaned animals. In adult animals, Na+, K+-ATPase and total ATPase partially diminished. GSH levels diminished significantly (p<0.05) both in weaned and in adult groups. The results indicate age-induced changes in cellular regulation and biochemical responses to oxidative stress induced by morphine.

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Acknowledgement

We thank to Isabel Pérez Monfort for hel** to translate the manuscript.

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Authors and Affiliations

  1. Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), CP 04530, Mexico D.F., Mexico

    David Calderón Guzmán, Ivonne Espítia Vázquez, Gerardo Barragán Mejía, Daniel Santamaría & Mario la Rosa de Apreza

  2. Laboratorio de Patología Experimental, Instituto Nacional de Pediatría (INP), CP 04530, Mexico D.F., Mexico

    Norma Osnaya Brizuela & Raquel García Alvarez

  3. Laboratorio de Farmacología, Instituto Nacional de Pediatría , Avenida Imán #1 3er piso, Colonia Cuicuilco, CP 04530, Mexico D.F., Mexico

    Ernestina Hernández García & Hugo Juárez Olguín

  4. Departamento de Farmacología, Facultad de Medicina, UNAM, Mexico, Mexico

    Hugo Juárez Olguín

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  1. David Calderón Guzmán
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  2. Ivonne Espítia Vázquez
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Correspondence to Hugo Juárez Olguín.

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Guzmán, D.C., Vázquez, I.E., Brizuela, N.O. et al. Assessment of Oxidative Damage Induced by Acute Doses of Morphine Sulfate in Postnatal and Adult Rat Brain. Neurochem Res 31, 549–554 (2006). https://doi.org/10.1007/s11064-006-9053-7

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  • DOI: https://doi.org/10.1007/s11064-006-9053-7

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