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The Pre-nervous Serotonergic System of Develo** Sea Urchin Embryos and Larvae: Pharmacologic and Immunocytochemical Evidence

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Abstract

Forty serotonin-related neurochemicals were tested on embryos and larvae of Lytechinus variegatus and other sea urchin species. Some of these substances (agonists of 5-HT1 receptors, antagonists of 5-HT2, 5-HT3 or 5-HT4 receptors, and inhibitors of the serotonin transporter, SERT) perturbed post-blastulation development, eliciting changes in embryonic/larval phenotypes typical for each class of receptor ligand. These developmental malformations were prevented completely or partially by serotonin (5-HT) or 5-HT analogs (5-HTQ, AA-5-HT), providing evidence for the putative localization of cellular targets. Immunoreactive 5-HT, 5-HT receptors and SERT were found in pre-nervous embryos and larvae of both L. variegatus and Strongylocentrotus droebachiensis. During gastrulation, these components of the serotonergic system were localized to the archenteron (primary gut), mesenchyme-like cells, and often the apical ectoderm. These results provide evidence that pre-nervous 5-HT may regulate early events of sea urchin embryogenesis, mediated by 5-HT receptors or the 5-HT transporter.

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  1. Robert E. Peterson

    Present address: Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, NC, 25799, USA

Authors and Affiliations

  1. Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC, 27599-7090, USA

    Gennady A. Buznikov, Lyudmila A. Nikitina & Jean M. Lauder

  2. Department of Biology, Coastal Carolina University, Conway, SC, USA

    Robert E. Peterson

  3. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117871, Moscow, Russia

    Vladimir V. Bezuglov

  4. N.K.Koltzov Institute of Developmental Biology, 117808, Moscow, Russia

    Gennady A. Buznikov

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  1. Gennady A. Buznikov
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  4. Vladimir V. Bezuglov
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  5. Jean M. Lauder
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Correspondence to Jean M. Lauder.

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Buznikov, G.A., Peterson, R.E., Nikitina, L.A. et al. The Pre-nervous Serotonergic System of Develo** Sea Urchin Embryos and Larvae: Pharmacologic and Immunocytochemical Evidence. Neurochem Res 30, 825–837 (2005). https://doi.org/10.1007/s11064-005-6876-6

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