Abstract
An eco-friendly microbial method for synthesis of silver colloid solution with antimicrobial activity is developed using a fungal strain of Penicillium purpurogenum NPMF. It is observed that increase in concentration of AgNO3 increases the formation of silver nanoparticle. At 5 mM concentration highly populated polydispersed nanoparticles form. Furthermore, change in pH of the reaction mixture leads to change in shape and size of silver nanoparticles. At lower pH two peaks are observed in the absorption spectra showing polydispersity of nanoparticles. However, highly monodispersed spherical nanoparticles of 8–10 nm size form with 1 mM AgNO3 concentration at pH 8. Antimicrobial activity of nanoparticles is demonstrated against pathogenic gram negative bacteria like Escherichia coli and Pseudomonas aeruginosa, and gram positive bacteria like Staphylococcus aureus. The antimicrobial activity of silver nanoparticles obtained at different initial pH show strong dependence on the surface area and shape of the nanoparticles.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M (2003) Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf B 28:313–318. doi:10.1016/S0927-7765(02)00174-1
Alivisatos AP (1996) Semiconductor clusters, nanocrystals, and quantum dots. Science 271:933–937. doi:10.1126/science.271.5251.933
Bae CH, Nam SH, Park SM (2002) Formation of silver nanoparticles by laser ablation of a silver target in NaCl solution. Appl Surf Sci 197:628–634. doi:10.1016/S0169-4332(02)00430-0
Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013–2016. doi:10.1126/science.281.5385.2013
Coe S, Woo WK, Bawendi M, Bulovic V (2002) Electroluminescence from single monolayer of nanocrystals in molecular organic devices. Nature 420:800–803. doi:10.1038/nature01217
Holmes JD, Smith PR, Evans-Gowing R, Richardson DJ, Russel DA, Sodeau JR (1995) Energy-dispersive-X-ray analysis of the extracellular cadmium sulfide crystallites of Klebsiella aerogenes. Arch Microbiol 163:143–147. doi:10.1007/BF00381789
Ingle A, Rai M, Gade A, Bawaskar M (2009) Fusarium solani: a novel biological agent for the extracellular synthesis of silver nanoparticles. J Nanopart Res 11:2079–2085. doi:10.1007/s11051-008-9573-y
Joerger KT, Joerger R, Olsson E, Granqvist CG (2001) Bacteria as workers in the living factory: metal-accumulating bacteria and their potential for materials science. Trends Biotechnol 19:15–20. doi:10.1016/S0167-7799(00)01514-6
Juan BH, Zhang ZM, Gong J (2006) Biological synthesis of semiconductor zinc sulfide nanoparticles by immobilized Rhodobacter sphaeroides. Biotechnol Lett 28:1135–1139. doi:10.1007/s10529-006-9063-1
Kalimuthu K, Babu RS, Venkataraman D, Bilal M, Gurunathan S (2008) Biosynthesis of silver nanocrystals by Bacillus licheniformis. Colloids Surf B 65:150–153. doi:10.1016/j.colsurfb.2008.02.018
Kathiresan K, Manivannan S, Nabeel MA, Dhivya B (2009) Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloids Surf B Biointerfaces 71:133–137. doi:10.1016/j.colsurfb.2009.01.016
Keki S, Torok J, Deak G, Daróczi L, Zsuga M (2000) Silver nanoparticles by PAMAM-assisted photochemical reduction of Ag+. J Colloid Interface Sci 229:550–553. doi:10.1006/jcis.2000.7011
Kowshik M, Vogel W, Urban J, Kulkarni SK, Paknikar KM (2002) Microbial synthesis of semiconductor PbS nanocrystallites. Adv Mater 14:815–818. doi:10.1002/1521-4095(20020605)
Kuber CB, D’Souza SF (2006) Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Colloids Surf B 47:160–164. doi:10.1016/j.colsurfb.2005.11.026
Lin SM, Lin FQ, Guo HQ, Zhang ZH, Wang ZG (2000) Surface states induced photoluminescence from Mn2+ doped CdS nanoparticles. Solid State Commun 115:615–618. doi:10.1016/S0038-1098(00)00254-4
Liu YC, Lin LH (2004) New pathway for the synthesis of ultrafine silver nanoparticles from bulk silver substrates in aqueous solutions by sonoelectrochemical methods. Electrochem Commun 6:1163–1168. doi:10.1016/j.elecom.2004.09.010
Mallick K, Witcomb MJ, Scurrell MS (2005) Self-assembly of silver nanoparticles in a polymer solvent: formation of a nanochain through nanoscale soldering. Mater Chem Phys 90:221–224. doi:10.1016/j.matchemphys.2004.10.030
Mandal D, Bolander ME, Mukhopadhyay D, Sarkar G, Mukherjee P (2006) The use of microorganisms for the formation of metal nanoparticles and their application. Appl Microbial Biotechnol 69:485–492. doi:10.1007/s00253-005-0179-3
Mann S (1996) Biomimetic materials chemistry. Wiley-VCH, New York
Marambio-Jones C, Hoek EMV (2010) A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res 12:1531–1551. doi:10.1007/s11051-010-9900-y
Mukherjee P, Senapati S, Mandal D, Ahmad A, Khan MI, Kumar R, Sastry M (2002) Extracellular synthesis of gold nanoparticles by the fungus Fusarium oxysporum. Chembiochem 3:461–463. doi:10.1002/1439-7633(20020503)
Narayanan KB, Sakthivel N (2010) Biological synthesis of metal nanoparticles by microbes. Adv Colloid Interface Sci 156:1–13. doi:10.1016/j.cis.2010.02.001
Pattabi M, Uchil J (2000) Synthesis of cadmium sulphide nanoparticles. Solar Energy Mater Solar Cell 63:309–314. doi:10.1016/S0927-0248(00)00050-7
Petit C, Lixon P, Pileni MP (1993) In situ synthesis of silver nanocluster in AOT reverse micelles. J Phys Chem 97:12974–12983. doi:10.1021/j100151a054
Ravindran TR, Arora AK, Balamurugan B, Mehta BR (1999) Inhomogeneous broadening in the photoluminescence spectrum of CdS nanoparticles. Nanostruct Mater 11:603–609. doi:10.1016/S0965-9773(99)00346-3
Sandmann G, Dietz H, Plieth W (2000) Preparation of silver nanoparticles on ITO surfaces by a double-pulse method. J Electroanal Chem 491:78–86. doi:10.1016/S0022-0728(00)00301-6
Shaligram NS, Bule M, Bhambure R, Singhal RS, Singh SK, Szakacs G, Pandey A (2009) Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain. Process Biochem 44:939–943. doi:10.1016/j.procbio.2009.04.009
Smetana AB, Klabunde KJ, Sorensen CM (2005) Synthesis of spherical silver nanoparticles by digestive ripening, stabilization with various agents, and their 3-D and 2-D superlattice formation. J Colloid Interface Sci 284:521–526. doi:10.1016/j.jcis.2004.10.038
Tan Y, Wang Y, Jiang L, Zhu D (2002) Thiosalicylic acid-functionalized silver nanoparticles synthesized in one-phase system. J Colloid Interface Sci 249:336–345. doi:10.1006/jcis.2001.8166
Vorobyova SA, Lesnikovich AI, Sobal NS (1999) Preparation of silver nanoparticles by interphase reduction. Colloids Surf A 152:375–379. doi:10.1016/S0927-7757(98)00861-9
Yu DG (2007) Formation of colloidal silver nanoparticles stabilized by Na+-poly (-glutamic acid) silver nitrate complex via chemical reduction process. Colloids Surf B 59:171–178. doi:10.1016/j.colsurfb.2007.05.007
Acknowledgments
Rati Ranjan Nayak would like to express his sincere thanks to Council of Scientific and Industrial Research (CSIR), India which gave an opportunity to perform research at Institute of Minerals and Materials Technology, Bhubaneswar under CSIR quick hire scheme.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Nayak, R.R., Pradhan, N., Behera, D. et al. Green synthesis of silver nanoparticle by Penicillium purpurogenum NPMF: the process and optimization. J Nanopart Res 13, 3129–3137 (2011). https://doi.org/10.1007/s11051-010-0208-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11051-010-0208-8