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Plant growth promoting Pseudomonas aeruginosa from Valeriana wallichii displays antagonistic potential against three phytopathogenic fungi

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Abstract

The soil nature and characterstics are directly related to the micro-organisms present, bio-mineralization process, plant type and thus having harmonius and interdependent relationships. Soil bacteria having antagonistic activity against phytopathogens, play an important role in root growth, overall plant growth and also their composition depends upon the plant species. Population explosion across globe has resulted in indiscriminate use of chemical fertilizers, fungicides and pesticides, thus posing serious risk to plant productivity and soil flora. Plant growth promoting rhizobacteria (PGPRs) are considered safer than chemical fertilizers as they are eco-friendly and sustain longer after colonization in rhizospheric soil. PGPRs are preferred as a green choice and acts as a superior biocontrol agents against phytopathogens. In the present study, a potential rhizobacteria, Pseudomonas aeruginosa (isolate-2) was isolated from the rhizosphere of a medicinal plant, Valeriana wallichi. The bacterial isolate exhibited qualitative tests for plant growth promoting determinatives. It was also subjected to in-vitro biocontrol activity against potential phytopathogens viz. Alternaria alternata, Aspergillus flavus and F. oxysporum. The antagonistic efficacy against F. oxysporum was 56.2% followed by Alternaria alternata to be 51.02%. The maximum inhibition of radial growth of F. oxysporum was 69.2%, Alternaria alternata (46.4%) and Aspergillus flavus (15%). The Pseudomonas aeruginosa exhibited plant growth promotion rhizobacterial activity which can be expoited as biofertilizers. This study deals with microbial revitalization strategy and offers promising solution as a biocontrol agent to enhance crop yield. Further, PGPRs research using the interdisciplinary approaches like biotechnology, nanotechnology etc. will unravel the molecular mechanisms which may be helpful for maximizing its potential in sustainable agriculture.

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  1. Harish Chandra, Pragati Kumari, and Ritesh Bisht have contributed equally

Authors and Affiliations

  1. Department of Medicinal and Aromatic Plant, High Altitude Plant Physiology Research Centre, Hemvati Nandan Bahuguna Garhwal (Central) University, Srinagar Garhwal, Uttarakhand, 246174, India

    Harish Chandra

  2. Department of Life Science, Singhania University, Jhunjhunu, Rajasthan, 333515, India

    Pragati Kumari

  3. Department of Biotechnology, H.N.B. Garhwal University (A Central University), Srinagar (Garhwal), Uttarakhand, 246174, India

    Ritesh Bisht & Saurabh Yadav

  4. Department of Forestry, Uttaranchal University, Arcadia Grant, Premnagar, Dehradun, Uttarakhand, 248007, India

    Rajendra Prasad

  5. Department of Botany and Microbiology, Gurukula Kangri Vishwavidayalaya, Haridwar, 249404, India

    Harish Chandra

  6. S-02, Scientist Hostel, Chauras campus, Srinagar Garhwal, Uttarakhand, 246174, India

    Pragati Kumari

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11033_2020_5676_MOESM1_ESM.jpg

Fig. S1 Phylogeny tree of amplified sequences of 16S rRNA gene of bacterial Isolate-2 with other Pseudomonas aeruginosa retrieved from NCBI GenBank (JPG 1060 kb)

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Chandra, H., Kumari, P., Bisht, R. et al. Plant growth promoting Pseudomonas aeruginosa from Valeriana wallichii displays antagonistic potential against three phytopathogenic fungi. Mol Biol Rep 47, 6015–6026 (2020). https://doi.org/10.1007/s11033-020-05676-0

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