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The protective effect of rosmarinic acid on hyperthermia-induced C2C12 muscle cells damage

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Abstract

High temperature will cause animal tissues or cells damage. Rosmarinic acid (RA) is a good antioxidant and health care product, but the roles of RA in muscle cells damage and the mechanisms which caused by high temperature is still unknown. In this study, the roles of RA on hyperthermia-induced apoptosis and damage of C2C12 muscle cells were investigated. C2C12 cells were cultured in medium with different concentration (0, 25, 50, 100 µM) RA and treated in 42 °C high temperature to induce cellular apoptosis and damage. Then, these cells were analyzed effect of different dose of RA on cells apoptosis and damage. The results indicated that RA has protective effect on heat-stress induced cellular damage, and the cells have the higher cell viability at the dose of 50 µM RA by MTT assay. Hochest33342/PI double staining showed that the cellular apoptosis of C2C12 cells were decreased in the presence of selected 50 µM RA. Malondialdehyde formation and reactive oxygen species levels were also decreased significantly, but cellular superoxide dismutase activity was increased significantly in the presence of RA even in the condition of 42 °C. Meanwhile, Caspase-3 mRNA expression, Caspase-3 activity, and Bax/Bcl-2 ratio were reduced significantly, but the mRNA expression of Hsp72 was increased significantly in those hyperthermia-induced C2C12 cells in the presence of 50 µM RA. Taken together, the results at least discovered that RA has protective effects on hyperthermia-induced cellular apoptosis and damage of muscle cells by change the expression of stress-genes and increasing intracellular antioxidant capability.

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Acknowledgments

This work was supported by the Fundamental Research Funds for the Central Universities (No. KYZ201413), National “Twelfth Five-Year” Plan for Science & Technology Support (2012BAD28B01), The Natural Science Foundation of Jiangsu Province (No. SBK201241530), Jiangsu postdoctoral research grants program (1302002B).

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  1. Hui-**a Li

    Present address: College of Animal Science and Technology, Nan**g Agricultural University, 1# Weigang, Nan**g, 210095, Jiangsu, China

Authors and Affiliations

  1. College of Animal Science and Technology, Nan**g Agricultural University, 1# Weigang, Nan**g, 210095, Jiangsu, China

    Kun-Lin Chen

  2. National Center of Meat Quality and Safety Control, Nan**g Agricultural University, Nan**g, 210095, China

    Hui-**a Li, **ng-Lian Xu & Guang-Hong Zhou

  3. Synergetic Innovation Center of Food Safety and Nutrition, Nan**g Agricultural University, Nan**g, 210095, China

    **ng-Lian Xu & Guang-Hong Zhou

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  1. Kun-Lin Chen
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  2. Hui-**a Li
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Correspondence to Hui-**a Li or Guang-Hong Zhou.

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Chen, KL., Li, HX., Xu, XL. et al. The protective effect of rosmarinic acid on hyperthermia-induced C2C12 muscle cells damage. Mol Biol Rep 41, 5525–5531 (2014). https://doi.org/10.1007/s11033-014-3429-6

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  • DOI: https://doi.org/10.1007/s11033-014-3429-6

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