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The over-expression of Chrysanthemum crassum CcSOS1 improves the salinity tolerance of chrysanthemum

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Abstract

Soil salinity represents a major constraint on plant growth. Here, we report that the over-expression of the Chrysanthemum crassum plasma membrane Na+/H+ antiporter gene CcSOS1, driven by the CaMV 35S promoter, improved the salinity tolerance of chrysanthemum ‘**ba’. In salinity-stressed transgenic plants, both the proportion of the leaf area suffering damage and the electrical conductivity of the leaf were lower in the transgenic lines than in salinity-stressed wild type plants. After a 6 day exposure to 200 mM NaCl, the leaf content of both chlorophyll (a+b) and proline was higher in the transgenic than in the wild type plants. The activity of both superoxide dismutase and peroxidase was higher in the transgenic than in the wild type plants throughout the period of NaCl stress. The transgenic plants had a stronger control over the ingress of Na+ into the plant, particularly with respect to the youngest leaves, and so maintained a more favorable K+/Na+ ratio. The result suggests that a possible strategy for improving the salinity tolerance of chrysanthemum could target the restriction of Na+ accumulation. This study is the first to report the transgenic expression of a Na+ efflux carrier in chrysanthemum.

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Acknowledgments

This study is support by the National Natural Science Foundation of China (31171987), 948 Project of Ministry of Agriculture (Grant No. 2011-G17), the Fundamental Research Funds for the Central Universities (KYZ201112), the Program for New Century Excellent Talents in University of Chinese Ministry of Education (Grant No. NCET-10-0492), Sci-Tech Support Plan of Jiangsu Province (BE2010303, BE2011325, BE2012350), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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  1. College of Horticulture, Nan**g Agricultural University, Nan**g, 210095, China

    Juan An, Ai** Song, Zhiyong Guan, Jiafu Jiang, Fadi Chen, Wanghuai Lou, Weimin Fang, Zhaolei Liu & Sumei Chen

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  1. Juan An
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An, J., Song, A., Guan, Z. et al. The over-expression of Chrysanthemum crassum CcSOS1 improves the salinity tolerance of chrysanthemum. Mol Biol Rep 41, 4155–4162 (2014). https://doi.org/10.1007/s11033-014-3287-2

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