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IiSDD1, a gene responsive to autopolyploidy and environmental factors in Isatis indigotica

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Abstract

In plants, stomata play a pivotal role in the regulation of gas exchange and are distributed throughout the aerial epidermis. SDD1, a gene isolated from Arabidopsis thaliana has been demonstrated to specialize in stomatal density and distribution. In our present study, a comprehensive survey of global gene expression performed by using an A. thaliana whole genome Affymetrix gene chip revealed SDD1 tends to be significantly lower in tetraploid Isatis indigotica than in diploid ones. To intensively investigate different SDD1 expression in response to polyploidy, a full-length cDNA clone (IiSDD1) encoding SDD1 was isolated from the traditional Chinese medicinal herb I. indigotica cDNA library. IiSDD1 shared a high level of identity with that from A. thaliana, containing some basic features of subtilases: D, H and S regions, as well as a substrate-binding site. Real-time quantitative PCR analysis indicated that IiSDD1 was constitutively expressed in all tested tissues, including roots, stems and leaves, both in tetraploid and diploid I. indigotica, and with the highest expression in leaves. In addition, IiSDD1 was also found to be down-regulated by signalling molecules for plant defence responses, such as abscisic acid (100 μM) and gibberellin (100 mg/L), as well as by environmental stresses including salt, darkness, coldness and drought. Our study, for the first time, indicates SDD1 participates not only in the defense/stress responsive pathways, but also probably involves in plants polyploidy evolution.

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Acknowledgments

This research was financially supported by National Natural Science Foundation of China (30600807); Modernization of traditional Chinese medicine foundation (08DZ1971502) and western development cooperation foundation (084358014), Shanghai Science and Technology Committee.

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Authors and Affiliations

  1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, 200003, Shanghai, People’s Republic of China

    Ying **ao, **ao**g Yu, Junfeng Chen, Peng Di & Wansheng Chen

  2. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People’s Republic of China

    Ying **ao & Lei Zhang

  3. Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, 200433, Shanghai, People’s Republic of China

    **ao**g Yu, Junfeng Chen & Wansheng Chen

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  1. Ying **ao
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  2. **ao**g Yu
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Correspondence to Wansheng Chen or Lei Zhang.

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Supplementary data 1

Nucleotide sequence and the deduced amino acid sequence of IiSDD1. The start codon (ATG) is in italics and the stop codon (TGA) is in bold. The typical domains of subtilisin-like serine proteases: three characteristic catalytic domains (D, H and S regions) and a substrate-binding site (N) are boxed. (GIF 49 kb)

Supplementary data 2

The 5′ flanking sequence of IiSDD1. The putative transcription initiation site A is shown in grey background. The most probable TATA box is boxed and in bold. The CAAT boxes are underlined. The cis-acting regulatory elements involved in abscisic acid responsiveness is underlined and in grey background. Three defense and stress responsive elements are in bold and in grey background. The light responsiveness-related elements are box, including 1 ACE, 2 AE-box, 1 Box I, 1 CATT-motif, 2 G-Box, 4 G-box, 1 GA-motif, 2 GAG-motif, 1 GT1-motif, 1 I-box, 1 Sp1, 3 TCT-motif. (GIF 34 kb)

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**ao, Y., Yu, X., Chen, J. et al. IiSDD1, a gene responsive to autopolyploidy and environmental factors in Isatis indigotica . Mol Biol Rep 37, 987–994 (2010). https://doi.org/10.1007/s11033-009-9776-z

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