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Novel and favorable QTL allele clusters for end-use quality revealed by introgression lines derived from synthetic wheat

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Abstract

Wheat quality is an important target trait. Previous studies mainly focus on storage protein, but their contribution to quality is partial, and most loci for quality are still undetected. Wild species of wheat are valuable resources for wheat improvement and introgression lines (ILs) are the ideal materials for detecting quantitative trait loci (QTL). In this study, a set of 82 BC5 F2-6 ILs, carrying a range of introgressed chromosome segments from a synthetic hexaploid wheat Am3 (Triticum carthlicum × Aegilops tauschii), was developed and genotyped with 170 microsatellite markers. QTL analysis was performed for 14 parameters, sodium dodecyl sulfate sedimentation volume, grain protein content (GPC), grain hardness and 11 mixograph parameters, associated with end-use quality of wheat, using the materials harvested in three environments. This led to the detection of 116 QTL, with c. 95% of the positive alleles contributed by Am3. Six important and novel genomic regions for bread-making quality were found on chromosomes 2D, 3A, 4A, 4B, 5A and 6A. These loci for bread-making quality showed pleiotropy and had large positive effects on several quality parameters with no or very weak negative effect on grain yield, thus demonstrating the value of synthetic wheat as a source of useful genetic variation for the improvement of bread wheat quality.

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Abbreviations

AACC:

American association of cereal chemists

ANOVA:

Analysis of variance

GH:

Grain hardness

GPC:

Grain protein content

HMW-GS:

High-molecular-weight glutenin subunit

IL:

Introgression line

LMW-GS:

Low-molecular-weight glutenin subunit

GNS:

Grain number per spike

K–S:

Kolmogorov–Smirnov

MLV:

Midline left of peak value

MLW:

Midline left of peak width

MPTi:

Midline peak time

MPV:

Midline peak value

MPW:

Midline peak width

MRV:

Midline right of peak value

MRW:

Midline right of peak width

MT:

Mixing tolerance

MTxV:

Midline time x = 8 min value

MTxW:

Midline time x = 8 width

NIRS:

Near-infrared reflectance spectroscopy

QTL:

Quantitative trait locus/loci

SDS–PAGE:

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

SNS:

Spikelet number/spike

SSD:

SDS sedimentation volume

SSR:

Simple sequence repeat

TGW:

Thousand grain weight

WS:

Weakening slope

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Acknowledgments

This work was supported by the National Basic Research Program of China (‘973’ grant 2004 CB117200 and 2010 CB125900). We would like to thank Dr. Zhonghu He, Chinese Academy of Agricultural Sciences, for help with mixograph analysis, and Dr. **anchun **a, Chinese Academy of Agricultural Sciences, and Steven Xu from USDA-ARS Biosciences Research Lab, Fargo, for revising the English.

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Authors and Affiliations

  1. Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Bei**g, 100081, China

    Yulian Li, Ronghua Zhou, ** Wang, **angzheng Liao & Jizeng Jia

  2. Institut National de la Recherche Agronomique (INRA), UMR 1095 GDEC-UBP, 63100, Clermont-Ferrand, France

    Gerard Branlard

  3. Institute of Crops Sciences, Shandong Academy of Agricultural Sciences, **an, 250100, China

    Yulian Li

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  1. Yulian Li
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  2. Ronghua Zhou
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Correspondence to Jizeng Jia.

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Li, Y., Zhou, R., Wang, J. et al. Novel and favorable QTL allele clusters for end-use quality revealed by introgression lines derived from synthetic wheat. Mol Breeding 29, 627–643 (2012). https://doi.org/10.1007/s11032-011-9578-6

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