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AB-QTL analysis in spring barley: III. Identification of exotic alleles for the improvement of malting quality in spring barley (H. vulgare ssp. spontaneum)

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Abstract

Malting quality is genetically determined by the complex interaction of numerous traits which are expressed prior to and, in particular, during the malting process. Here, we applied the advanced backcross quantitative trait locus (AB-QTL) strategy (Tanksley and Nelson, Theor Appl Genet 92:191–203, 1996), to detect QTLs for malting quality traits and, in addition, to identify favourable exotic alleles for the improvement of malting quality. For this, the BC2DH population S42 was generated from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum). A QTL analysis in S42 for seven malting parameters measured in two different environments yielded 48 QTLs. The exotic genotype improved the trait performance at 18 (37.5%) of 48 QTLs. These favourable exotic alleles were detected, in particular, on the chromosome arms 3HL, 4HS, 4HL and 6HL. The exotic allele on 4HL, for example, improved α-amylase activity by 16.3%, fermentability by 0.8% and reduced raw protein by 2.4%. On chromosome 6HL, the exotic allele increased α-amylase by 16.0%, fermentability by 1.3%, friability by 7.3% and reduced viscosity by 2.9%. Favourable transgressive segregation, i.e. S42 lines exhibiting significantly better performance than the recurrent parent Scarlett, was recorded for four traits. For α-amylase, fermentability, fine-grind extract and VZ45 20, 16, 2 and 26 S42 lines, respectively, surpassed the recurrent parent Scarlett. The present study hence demonstrates that wild barley does harbour valuable alleles, which can enrich the genetic basis of cultivated barley and improve malting quality traits.

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Acknowledgements

We thank Dr. Eberhard Laubach (Nordsaat Saatzucht) and his team for conducting the field experiment at Gudow. We are greatly indebted to Dr. Lissy Kuntze (Nordsaat Saatzucht, Böhnshausen) for the analysis of malting parameters. The excellent technical assistance of Merle Noschinski, Carsten Golletz, and the team of the Dikopshof Research Station in Wesseling is appreciated. This work was funded by the German Plant Genome Research Initiative (GABI) of the Federal Ministry of Education and Research (BMBF, project 0312278A).

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  1. Maria von Korff

    Present address: International Center for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria

  2. Huajun Wang

    Present address: Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Agricultural University, Yingmencun 1, Anningqu district, Lanzhou, 730070, China

Authors and Affiliations

  1. Institute of Crop Science and Resource Conservation, University of Bonn, Katzenburgweg 5, 53115, Bonn, Germany

    Maria von Korff, Huajun Wang, Jens Léon & Klaus Pillen

  2. Max-Planck-Institute for Plant Breeding Research, Barley Genetics Research Group, Carl-von-Linné-Weg 10, 50829, Koln, Germany

    Klaus Pillen

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  1. Maria von Korff
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Correspondence to Klaus Pillen.

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von Korff, M., Wang, H., Léon, J. et al. AB-QTL analysis in spring barley: III. Identification of exotic alleles for the improvement of malting quality in spring barley (H. vulgare ssp. spontaneum). Mol Breeding 21, 81–93 (2008). https://doi.org/10.1007/s11032-007-9110-1

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