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CLDN-1 promoted the epithelial to migration and mesenchymal transition (EMT) in human bronchial epithelial cells via Notch pathway

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Abstract

Claudin-1 (CLDN-1) is one of main tight junction components that play an important role in epithelial–mesenchymal transition (EMT). However, the effects of CLDN-1 on the migration and EMT induced by TGF-β1 in primary normal human bronchial epithelial (NHBE) and BEAS-2B cells have not been clear. The expression of CLDN-1 was quantified by Western blotting in NHBE and BEAS-2B cells. Cell migration and invasion were detected using transwell assays. The expression level of E-cadherin, N-cadherin, α-SMA, and Vimentin was evaluated by quantitative real-time PCR and Western blotting. Here we showed that the protein expression of CLDN-1 was increased exposed to TGF-β1 in a dose- and time-dependent manner. Knockdown of CLDN-1 using small interfering CLDN-1 RNA (siCLDN-1) prevented the migration and invasion in NHBE and BEAS-2B cells. Moreover, depletion of CLDN-1 promoted the E-cadherin expression and decreased the mRNA and protein levels of N-cadherin, α-SMA, and Vimentin induced by TGF-β1. Furthermore, CLDN-1 silencing resulted in the reduction of the Notch intracellular domain (NICD) and hairy enhancer of split-1 (Hes-1) in mRNA and protein level. Jagged-1, an activator of Notch signaling pathway, abrogated the protective function of siCLDN-1 in migration and EMT. In conclusion, CLDN-1 promoted the migration and EMT through the Notch signaling pathway.

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Authors and Affiliations

  1. Department of Respiration Medicine, Cangzhou Central Hospital, Cangzhou, 061001, People’s Republic of China

    **g Lv, Baohua Sun, Zhitao Mai, Mingming Jiang & Junfeng Du

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  1. **g Lv
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  2. Baohua Sun
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  3. Zhitao Mai
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  4. Mingming Jiang
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Correspondence to Baohua Sun.

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Lv, J., Sun, B., Mai, Z. et al. CLDN-1 promoted the epithelial to migration and mesenchymal transition (EMT) in human bronchial epithelial cells via Notch pathway. Mol Cell Biochem 432, 91–98 (2017). https://doi.org/10.1007/s11010-017-3000-6

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  • DOI: https://doi.org/10.1007/s11010-017-3000-6

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